壁虎活性组分对HepG2细胞增殖及活性氧的影响  

Effects of gecko active components on proliferation and reactive oxygen species in HepG2 cells

作　　者：段一梦 段冷昕[1] 刘玲[1] 郭梦丽 王兵兵 黄泽月 王建刚[1] DUAN Yi-meng;DUAN Leng-xin;LIU Ling;GUO Meng-li;WANG Bing-bing;HUANG Ze-yue;WANG Jian-gang(The Key Laboratory of Pharmacology and Medical Molecular Biology, Medical College, Henan University of Science arm Technology, Luoyang 471023, Henan Province, China)

机构地区：[1]河南科技大学医学院药理学与分子生物学实验室,河南洛阳471023

出　　处：《中国临床药理学杂志》2018年第8期964-967,共4页The Chinese Journal of Clinical Pharmacology

基　　金：河南省科技重点攻关基金资助项目(142102310031)

摘　　要：目的该研究旨在探讨壁虎活性组分(GACs)对人肝癌HepG2细胞内活性氧水平,钙离子水平及线粒体膜电位水平的影响。方法实验分为对照组(不加药)和低、中、高3个浓度实验组(GACs,0.1,0.2,0.3 mg·m L^(-1))。GACs作用于HepG2细胞24 h后,用噻唑蓝比色法(MTT法)检测GACs对HepG2细胞增殖能力的影响,用流式细胞术检测细胞内活性氧(ROS)水平、钙离子水平及线粒体膜电位(MMP)水平。结果 GACs可抑制HepG2细胞的增殖,并具有质量浓度依赖性,作用24 h后其IC_(50)值为0.21 mg·m L^(-1)。对照组、低、中、高剂量组细胞增殖抑制率分别为(0.07±0.01)%,(0.17±0.03)%,(0.49±0.12)%和(0.67±0.18)%;ROS含量分别为(16.61±1.12),(85.81±2.56),(268.91±2.34),(1741.5±5.64);钙离子水平分别为(9.67±0.98),(12.30±1.07),(94.80±2.75),(910.99±5.31);MMP分别为(27.02±1.8)×10^(-2),(23.78±1.2)×10^(-2),(18.27±1.5)×10^(-2),(16.49±2.1)×10^(-2),低、中、高剂量组各指标分别与对照组比较,差异均有统计学意义(P<0.05或P<0.01)。结论 GACs能抑制HepG2细胞增殖,可能通过引起HepG2细胞内ROS含量升高、钙离子水平升高、MMP降低,扰乱线粒体的正常功能,最终导致HepG2细胞的凋亡。Objective The aim of this study was to investigate the effects of gecko active components（ GACs） on the levels of reactive oxygen species（ ROS）, calcium and mitochondrial membrane potential（ MTP） in HepG2 cells. Methods The experiment was divided into 4 groups： control group（ GACs,0）,low,middle and high dose group（ 0. 1,0. 2,0. 3 mg·m L-（-1）,GACs）. The growth inhibitory effect of GACs on HepG2 cells were assessed by 3-（ 4,5-dimethylthiazol-2 yl）-2,5-diphenyltetrazolium bromide（ MTT） assay. Intracellular reactive oxygen species（ ROS） level,calcium level,mitochondrial membrane potential（ MMP） level was measured by flow cytometry. Results The results showed that GACs could inhibit the proliferation of HepG2 cells in a dose-dependent manners. After treatment of GACs for 24 h,the 50%inhibitory dose（ IC（50）） values were 0. 21 mg·m L-（-1） The cell growth inhibition rates of the control group,low-,middle-and high-dose groups were（ 0. 07 ± 0. 01） %,（ 0. 17 ± 0. 03） %,（ 0. 49 ± 0. 12） % and（ 0. 67 ± 0. 18） %,respectively; the ROS levels of control group,low-,middle-and high-dose groups were（ 16. 61 ± 1. 12）,（ 85. 81 ± 2. 56）,（ 268. 91 ± 2. 34）,（ 1741. 5 ± 5. 64）; the calcium levels of control group,low,middle and high-dose groups were（ 9. 67 ± 0. 98）,（ 12. 30 ± 1. 07）,（ 94. 80 ± 2. 75）,（ 910. 99 ± 5. 31）; the MMP levels of control group,low-,middle-and high-dose groups were（ 27. 02 ± 1. 8） × 10-（-2）,（ 23. 78 ± 1. 2） × 10-2,（ 18. 27 ± 1. 5） × 10-（-2）,（ 16. 49 ± 2. 1） × 10-（-2）,and significant differences were found in the above indexes between low/middle/high dose group and the control group（ P〈0. 05 or P〈0. 01）. Conclusion GACs can inhibit the proliferation of HepG2 cells,which may cause the increase of ROS content and calcium level and the decrease of MMP,and disrupt the normal function of mitochondria,eventually leading to the apoptosis of HepG2 cells.

关 键 词：壁虎活性组分 活性氧 钙离子 线粒体膜电位 

分 类 号：R28[医药卫生—中药学]