幼年小鼠初级听皮层第V层锥体神经元持续性放电与毒蕈碱受体亚型关系  

Subtypes of muscarinic acetylcholine receptors involved in the persistent activity of layer V pyramidal neurons in the primary auditory cortex of young mice

作　　者：贾慧娟 王欣[1] 付欣 杜小凤 郑维维 罗峰[1] JIA Hui-Juan;WANG Xin;FU Xin;DU Xiao-Feng;ZHENG Wei-Wei;LUO Feng(School of Life Sciences and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan 430079, Chin)

机构地区：[1]华中师范大学生命科学学院湖北省遗传调控与整合生物学重点实验室,武汉430079

出　　处：《生理学报》2018年第2期123-131,共9页Acta Physiologica Sinica

基　　金：supported by the National Natural Science Foundation of China(No.31300915);the Scientific Research Project Fund for Youth Teachers from Central China Normal University(No.CCNU15A05035)

摘　　要：激活胆碱能受体结合去极化电流诱导产生的持续性放电(persistent activity,PA)是神经元产生可塑性的一种特征表现。小鼠初级听皮层(primary auditory cortex,AI)神经元PA的产生与毒蕈碱受体(即M型受体)密切相关,但其涉及的毒蕈碱受体亚型尚不清楚。本研究采用离体脑片全细胞膜片钳技术结合药理学方法,探讨幼年小鼠AI第V层锥体神经元PA与毒蕈碱受体亚型之间的关系。结果显示,激活胆碱能受体并同时注入去极化电流可诱导锥体神经元产生PA。M1、M2和M3受体拮抗剂分别能阻断PA的产生,而M4受体拮抗剂对PA的产生没有影响。该结果表明M1、M2和M3毒蕈碱受体均参与幼年小鼠AI第V层锥体神经元PA的形成,而M4毒蕈碱受体不参与PA的形成。Cholinergic receptor activation and intracellular current injection lead to the persistent activity（PA）, which may be involved in inducing neural plasticity. Our previous study showed that PA is closely related to the activation of muscarinic acetylcholine receptors（mAChRs） in pyramidal neurons of mouse primary auditory cortex（AI）. However, the subtypes of mAChRs involved in PA remain unclear. Thus, using whole-cell patch-clamp recording and pharmacological methods, we investigated the role of different mAChR subtypes in inducing PA in AI layer V pyramidal neurons of young mice. The results showed that activation of mAChRs with intracellular depolarizing current induced PA in layer V pyramidal neurons. Blockade of M1, M2 or M3 subtypes prevented the PA, whereas M4 receptor antagonists did not affect the production of PA. The results suggest that the PA may be induced through a mechanism involving M1, M2 and M3 muscarinic receptors, but not M4 subtype.

关 键 词：全细胞膜片钳 毒蕈碱受体 持续性放电 可塑性 小鼠 

分 类 号：Q437[生物学—生理学] R329[医药卫生—人体解剖和组织胚胎学]