基于单棱镜复用的轴向可调式共光路干涉相位成像系统  

Axial Adjustable Common-Path Interferometric Phase Imaging System Based on Prism Reuse

作　　者：张明明[1] 韩豪 刘景业 徐媛媛[2] 王亚伟[2] 季颖[2] Zhang Mingming;Han Hao;Liu Jingye;Xu Yuanyuan;Wang Yawei;Ji Ying(School of Mechanical Engineering, Jiangsu University, Zhenjiang , Jiangsu 212013, China;Faculty of Science, Jiangsu University, Zhenj iang , Jiangsu 212013, China)

机构地区：[1]江苏大学机械工程学院,江苏镇江212013 [2]江苏大学理学院,江苏镇江212013

出　　处：《中国激光》2018年第4期295-302,共8页Chinese Journal of Lasers

基　　金：国家自然科学基金(11474134;11374130;11604127);江苏省"六大人才高峰"(2015-DZXX-023)

摘　　要：基于干涉成像原理,提出了一种结构简单的轴向可调式共光路干涉成像系统的设计。该设计充分利用光路可逆性和分光棱镜的反射、折射特性,先通过棱镜将入射光分为两束平行光,一束照射到样品上被反射载物台反射作为物光,另一束则直接被反射作为参考光,然后利用上述棱镜使反射回的物光和参考光合束发生干涉。通过调整棱镜的分光层与光轴之间的夹角可分别实现不同类型的干涉。分别以傅里叶变换和三步相移范数法为例对实验获得的干涉条纹进行相位恢复,并通过对结果的分析评估了系统的性能,对设计用于生物细胞相位成像的可行性进行了论证。所设计的系统结构简单、操作方便、系统误差小,可为均质细胞的无损免标记形态检测提供一种简单易行的方法。An axial adjustable common-path interferometric phase imaging system with simple structure is proposed based on the theory of interference imaging.In this design,making full use of the reversibility of the optical path and the reflection and refraction characteristics of the splitter prism,the incident beam is split into two parallel beams by aprism.One beam is incident on the sample and reflected by the reflecting stage as object light,and the other beam is directly reflected as reference light.The interferograms can be captured by combining object beam with reference beam through the same beam splitter.The interferometer can achieve different types of interference by adjusting the angle between the layer of the beam splitter prism and the optic axis.Taking Fourier transform and three steps phase shift norm method as examples,the phase of the interference fringe is recovered,and the performance of the system is evaluated by the analysis of the results.The feasibility of the proposed design for biological cells phase imaging is demonstrated.The system has the characteristics of simple structure,easy operation and low system error,which can provide an easy and simple method for label-free morphological detection of homogeneous cell.

关 键 词：生物光学 相位成像 干涉系统 共光路 生物细胞 

分 类 号：O436.1[机械工程—光学工程]