酶联免疫吸附法测定儿童血清中双酚A含量  

Detection of serum bisphenol A with enzyme-linked immunosorbent assay among children

作　　者：许龙 章英[2] 朱立鑫 杨帆帆 赖肖[2] 孟玮 范艳 刘仁荣 XU Long;ZHANG Ying;ZHU Li-xin;et al(College of Life Science, Jiangxi Science and Technology Normal University, Nanchang, Jiangxi Province 330013, China)

机构地区：[1]江西科技师范大学生命科学学院,江西南昌330013 [2]南昌市疾病预防控制中心公共卫生监测所

出　　处：《中国公共卫生》2018年第4期593-596,共4页Chinese Journal of Public Health

基　　金：国家自然科学基金(81360429);江西省高等学校科技落地计划(GJJ13573/13574);南昌市科技计划项目(洪财企[2012];37号-23)

摘　　要：目的应用抗双酚A单克隆抗体3H1,建立了间接竞争酶免疫吸附法(ELISA)检测血清中双酚A(BPA)浓度。方法按照间接竞争酶联免疫吸附法,2014年选取江西省南昌地区儿童人群作为研究对象,测定其血清中BPA浓度水平。结果该ELISA方法的检测限为0.43 ng/m L,血清的加标回收率为70.1%~87.8%,变异系数为4.79%~9.41%。176份血清样品中,95份可检出BPA,检出率为54%。全部样品的BPA的浓度范围为未检出(N.D.)至26.48 ng/m L。男童检出率高于女童,检出率在不同年龄组间差异不明显(P=0.195)。结论建立的ELISA方法能够满足检测儿童人群血清中BPA的要求。江西南昌地区儿童人群中血清中存在BPA污染,个别污染水平相对较高,需关注其来源。Objective To establish an indirect competitive enzyme-linked immunosorbent assay （ic-ELISA） using monoclonal antibody of bisphenol A （BPA） for the detection of serum BPA.Methods Totally 176 pupils aged 8-10 years were selected from three primary schools in suburban area of Nanchang city of Jiangxi province and serum BPA of the pupils were determined with ic-ELISA established.Results The detection limit of the established ic-ELISA method was 0.43 ng/mL. The recovery rates of BPA in serum spiked at 4 different levels were between 70.1% and 87.8% with the variation coefficients from 4.79% to 9.41%. BPA was detected in 95 of the 176 serum samples and the detection rate was 54%. The serum BPA content ranged from non-detectable to 26.48 ng/mL for all the samples. The detection rate of serum BPA was higher among the boy pupils than among the girl pupils, but the detection rate was not significantly different among the pupils of various age groups （P=0.195）.Conclusion The established ic-ELISA could meet the requirement of serum BPA detection among children. BPA was detectable in serum of children in Nanchang city of Jiangxi province and relatively higher serum BPA content was detected in some of the children, suggesting studies are needed to explore the contamination source of BPA.

关 键 词：酶免疫吸附法 血清中双酚A(BPA)水平 儿童人群 

分 类 号：TQ323.5[化学工程—合成树脂塑料工业]