库布齐沙地生物土壤结皮中解磷菌的分离鉴定及解磷能力  

Identification and isolation of phosphorus-solubilizing bacteria of biological soil crusts in Kubqi sandland and their phosphorus-solubilizing capability

作　　者：贾丽娟 唐凯 高晓丹 徐慧欣 李蘅 孟建宇 袁立敏[2] 冯福应 JIA Lijuan;TANG Kai;GAO Xiaodan;XU Huixin;LI Heng;MENG Jianyu;YUAN Limin;FENG Fuying(Institute for Applied ＆ Environmental Microbiology, College of Life Sciences, Inner Mongolia Agricultural University, Huhhot 010018, China;Inner Mongolia Academy of Forestry Science, Huhhot 010010, China)

机构地区：[1]内蒙古农业大学生命科学学院应用与环境微生物研究所,呼和浩特010018 [2]内蒙古自治区林业科学研究院,呼和浩特010010

出　　处：《应用与环境生物学报》2018年第2期390-394,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(31560030);国家科技支撑计划项目(2015BAC06B01);内蒙古自治区高等学校青年科技英才支持计划项目(NJYT-14-A05);内蒙古农业大学生命科学学院师生协同科技创新项目(KJCX2016011)资助~~

摘　　要：解磷菌对生物土壤结皮（Biological soil crusts，BSCs）中不同形态磷之间的转化起重要的生物驱动作用. 以无机磷和有机磷培养基分离筛选库布齐沙地BSCs中的解磷菌，基于16S rRNA基因进行分类鉴定；采用钼锑钪比色法测定菌株解无机磷能力；采用钒钼比色法测定其解植酸磷能力，以植酸酶活性表示. 结果共分离得到33株可在无机磷（磷酸钙）培养基上生长迅速的细菌，以Bacillus属和Sphingomonas属为主；其中18株菌可以使PVK培养基变色，解无机磷能力较强，达0.422-3.531 mg/mL；这18株菌中有15株可在植酸钙培养基上生长，其植酸酶活力为2.45-20.84 IU/mL，主要为Sphingomonas属.Phosphate-solubilizing bacteria （PSB） play a fundamental biological role in the transformation of different phosphorus forms in biological soil crusts （BSCs）. To investigate the community composition of PSB in BSCs of Kubqi sandland and capability to dissolve phosphorus, inorganic and organic phosphorus medium was used to isolate phosphorus-solubilizing bacteria. A preliminary identification was based on the 16S rRNA gene; spectrophotometry of molybdate-antimony-scandium and vanadium-ammonium-molybdate were used to measure the inorganic and organic phosphorus-solubilizing capabilities, respectively; the organic phosphorus-solubilizing ability was indicated by phytase activity. The results showed that a total of 33 phosphorus bacteria were isolated from BSCs in Kubqi sandland, which grew rapidly on inorganic phosphorus medium, and were dominated by Bacillus and Sphingomonas; among them, 18 strains changed the color of PVK medium with inorganic phosphorus solubilizing capability of 0.422–3.531 mg/mL; 15 of the 18 strains grew on phytate-Ca medium with phytase activity of 2.45–20.84 IU/mL, and Sphingomonas was dominant. This study preliminarily revealed the composition and ability of PSB in BSCs of Kubqi sandland, which provides a basis for understanding and using desert PSB for other applications.

关 键 词：生物土壤结皮 解磷菌 磷酸钙 植酸磷 16S RDNA 

分 类 号：S154.39[农业科学—土壤学]