HPLC同时测定独一味丸中两种活性成分的含量  

Simultaneous Determination of Two Active Components in Lamiophlomis Rotata Pill by HPLC

作　　者：郝自新 陈庆文 杨积慧 程世云 HAO Zi-xin;CHEN Qing-wen;YANG Ji-hui;CHENG Shi-yun(Anqing Testing Center for Food and Drug,Anqing 24600：1, China;Anqing Food and Drug Inspection Detachment, Anqing 24600：1, China;Anhui Institute for Food and Drug Control, Hefei 2300.5：1, China)

机构地区：[1]安庆市食品药品检验中心,安庆246001 [2]安庆市食品药品稽查支队,安庆246001 [3]安徽省食品药品检验研究院,合肥230051

出　　处：《海峡药学》2018年第4期77-79,共3页Strait Pharmaceutical Journal

摘　　要：目的建立同时测定独一味丸中两种活性成分的高效液相色谱分析方法。方法采用Waters XBridge C18(250mm×4.6mm,5μm)色谱柱,以乙腈(A)-水(B)为流动相,梯度洗脱(0~11min,9%A;11~14min,9%A→15%A;14~35min,15%A),流速:1.0m L·min-1,检测波长为235 nm;柱温:35℃。结果山栀苷甲酯在0.03940~0.7880μg之间线性关系良好,r=0.9997,8-O-乙酰山栀苷甲酯在0.04791~0.9582μg之间线性关系良好,r=0.9999,山栀苷甲酯的平均回收率为99.30%,RSD=1.10%,8-O-乙酰山栀苷甲酯的平均回收率为99.17%,RSD=1.02%。结论该方法操作简单,重复性好,准确度高,分离效果好,可以用于独一味丸的质量控制。OBJECTIVE To establish a method for simultaneous determination of two active components in Lamiophlomis rotata pill by HPLC. METHODS The separation was performed on a Waters XBridge Cls （4. 6mm × 250mm,5μm） column, using aeetonitrile（A） -water（B） as the mobile phase in a gradient mode （ 0 1 lmin ,9% A ; 11 - 14min,9% A-＋15 % A; 14 - 35 min, 15 % A） at a flow rate of 1.0mL· min- 1, the detection wavelengths were 235nm,with the column temperature of 30℃. RESULTS Shanzhiside methyl ester in 0. 03940 -0. 7880μg between a good linear relationship, r = 0. 9997 ;8-O-acetyl shanzhiside methyl ester in 0. 04791 -0. 9582μg between a good linear relationship, r -- 0. 9999. The average recovery rate of Shanzhiside methyl ester was 99.30%, RSD = 1.10% ,the average recovery rate of 8-O-acetyl shanzhiside methyl ester was 99. 17% RSD = 1.02%. CONCLUSION This method has the advantages of simple operation, good repeatability, high accuracy, good separation effect, can he used in Lamiophlomis rotata pill quality control.

关 键 词：高效液相色谱法 独一味丸 山栀苷甲酯 8-O-乙酰山栀苷甲酯 

分 类 号：R927[医药卫生—药学]