哮喘-慢阻肺重叠综合征与慢阻肺患者中性粒细胞明胶酶相关脂质运载蛋白的差异性研究  

A study on the differences in neutrophil gelatinase-associated lipocalin levels in patients with chronic obstructive pulmonary disease and patients with asthma-chronic obstructive pulmonary disease overlap syndrome

作　　者：李晓平[1] 刘冀[1] 杨发满[1] 汪元浚[1] 李蓉[1] 张培莉[1] 王卓亚[1] LI Xiao-ping;LIU Ji;YANG Fa-man;WANG Yuan-jun;LI Rong;ZHANG Pei-li;WANG Zhuo-ya(Geriatrics, Qinghai University Hospital, Qinghai, Xining 810000, China)

机构地区：[1]青海大学附属医院老年病科,青海西宁810000

出　　处：《中国病原生物学杂志》2018年第3期306-310,共5页Journal of Pathogen Biology

基　　金：青海大学附属医院中青年科研基金项目(No.ASRF-2014-)

摘　　要：目的探讨哮喘-慢阻肺重叠综合征(ACOS)与慢性阻塞性肺病(COPD)患者中性粒细胞明胶酶相关脂质运载蛋白(NGAL)的差异性。方法随机选取急性加重期和稳定期COPD患者,急性加重期和稳定期ACOS患者以及健康对照各30例,采用酶联免疫吸附试验(ELISA)检测血和痰液NGAL水平,采用person相关分析法分析其与中性粒细胞百分比、FEV1占预计值百分比(%)和血液C反应蛋白(C-reactive protein,CRP)含量的相关性。结果急性加重期和稳定期COPD患者,急性加重期和稳定期ACOS患者以及健康对照血NGAL浓度分别为(119.29±16.42)g/L、(79.06±13.25)g/L、(126.95±19.67)g/L、(71.01±9.36)g/L、(62.11±13.01)g/L,痰液NGAL浓度分别为(16.1±4.9)g/L、(2.9±0.7)g/L、(15.8±5.2)g/L、(3.2±0.8)g/L、(0.6±0.4)g/L,各组间比较差异均有统计学意义(P<0.05),其中急性加重期的患者血NGAL高于稳定期及健康对照组(P<0.05)。血NGAL与COPD和ACOS急性加重期和稳定期血液CRP含量、中性粒细胞百分比呈正相关(P<0.05),而与白细胞总数无显著相关性(P>0.05);血NGAL与COPD以及ACOS急性加重期FEV1占预计值百分比(%)呈负相关(P<0.05),与稳定期FEV1占预计值百分比(%)呈正相关(P<0.05)。痰液NGAL与COPD以及ACOS急性加重期和稳定期痰液中性粒细胞百分比呈正相关(P<0.05),与FEV1占预计值百分比(%)呈负相关(P<0.05)。结论COPD及ACOS不同期患者血液及痰液NGAL水平不同程度增高,可作为COPD及ACOS患者病情严重程度的指标。Objective To explore differences in neutrophil gelatinase-associated lipocalin（NGAL）levels in patients with asthma-chronic obstructive pulmonary disease overlap syndrome（ACOS）and chronic obstructive pulmonary disease（CPOD）. Methods Subjects were 30 randomly selected patients with acute exacerbated or stable COPD and 30 patients with acute exacerbated or stable ACOS.An enzyme-linked immunosorbent assay（ELISA）was used to detect NGAL levels in plasma and sputum,and a Pearson analysis was used to analyze the correlation between levels of NGAL and the percentage of neutral granulocytes,the estimated percentage of FEV1（%）,or the level of CRP in the blood.Results：The level of NGAL in the blood was 1,119.29±16.42 g/L in patients with acute exacerbated COPD,79.06±13.25 g/L in patients with stable COPD,126.95±19.67 g/L in patients with acute exacerbated ACOS,71.01±9.36 g/L in patients with stable ACOS,and 62.11±13.01 g/L in healthy controls.The level of NGAL in sputum was 16.1±4.9 g/L in patients with acute exacerbated COPD,2.9±0.7 g/L in patients with stable COPD,15.8±5.2 g/L in patients with acute exacerbated ACOS,3.2±0.8 g/L in patients with stable ACOS,and 0.6±0.4 g/L in healthy controls.Levels of NGAL in the blood and serum differed significantly among the groups（P〈0.05）.The level of NGAL in the blood of patients with acute exacerbated COPD or ACOS was higher than that in patients with stable COPD or ACOS and that in healthy controls（P〈0.05）.Moreover,the level of NGAL in the blood was correlated with the level of CRP and the percentage of neutral granulocytes in acute exacerbated COPD and ACOS（P〈0.05）,but it was not correlated with the white blood cell count（P〈0.05）.The level of NGAL in the blood was inversely correlated with the estimated percentage of FEV1（%）in patients with acute exacerbated COPD or ACOS,and it was correlated with the estimated percentage of FEV1（%）in patients with stable COPD or ACOS（P〈0.05）.The level of NGAL in sputum was corre

关 键 词：哮喘-慢阻肺重叠综合征 慢性阻塞性肺病 中性粒细胞明胶酶相关脂质运载蛋白 差异性 

分 类 号：R562.5[医药卫生—呼吸系统]