新仙灵骨葆胶囊对大鼠骨折愈合的影响研究  

Study on the Effects of New Xianling Gubao Capsule on Fracture Healing in Rats

作　　者：刘明[1] 刘杨[1] 张永萍 徐剑 孙宜春 白志文 LIU Ming;LIU Yang;ZHANG Yongping;XU Jian;SUN Yichun;BAI Zhiwen(Basic Medical School, Guiyang College of TCM, Guiyang 550025, China;School of Pharmacy, Guiyang College of TCM, Guiyang 550025, China;Sinopharm Group Tongjitang（Guizhou）Pharmaceutical Co., Ltd., Guiyang 550009, China)

机构地区：[1]贵阳中医学院基础医学院,贵阳550025 [2]贵阳中医学院药学院,贵阳550025 [3]国药集团同济堂(贵州)制药有限公司,贵阳550009

出　　处：《中国药房》2018年第9期1201-1204,共4页China Pharmacy

基　　金：国家科技重大专项项目(No.2011ZX09201-201-05);贵州省协同创新中心建设项目(No.黔教合协同创新字[2013]02号);贵州省普通高等学校工程研究中心建设项目(No.黔教合KY字[2014]22号)

摘　　要：目的:研究新仙灵骨葆胶囊对大鼠骨折愈合的影响,为寻求"减量增效(减量等效)"新组方提供参考。方法:将大鼠随机分为假手术组(蒸馏水)、模型组(蒸馏水)、仙灵骨葆胶囊组(350 mg/kg)和新仙灵骨葆胶囊低、中、高剂量组(53、105、210 mg/kg),每组14只。除假手术组外,其余各组大鼠均在麻醉状态下制作右侧股骨中段骨折模型。待大鼠术后清醒,各组大鼠灌胃相应药物10 m L/kg,每天给药1次,连续4周。末次给药后,测定大鼠体质量,观察骨折处骨痂形成及组织病理学变化,进行骨折侧生物力学(骨折应力、骨压碎力)测量,并采用酶联免疫吸附法检测血清中炎症因子[肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)]水平。结果:与假手术组比较,模型组大鼠体质量和骨折侧骨折应力、骨压碎力明显降低(P<0.05或P<0.01),血清中TNF-α、IL-1β水平明显升高(P<0.01);模型组大鼠骨折周围可见明显骨痂形成,骨小梁不成熟、排列混乱。与模型组比较,新仙灵骨葆胶囊高剂量组和仙灵骨葆胶囊组大鼠体质量明显增加(P<0.05),新仙灵骨葆胶囊中、高剂量组和仙灵骨葆胶囊组大鼠骨折侧骨折应力明显升高(P<0.05或P<0.01),各给药组大鼠血清中TNF-α、IL-1β水平均明显降低(P<0.01);且新仙灵骨葆胶囊各剂量组与仙灵骨葆胶囊组比较上述指标差异均无统计学意义(P>0.05)。各给药组大鼠骨痂面积均变小,骨折线变模糊,骨折痕迹基本消失,骨小梁数量增多;骨折处皮质层增厚,骨髓腔见大量毛细血管植入。结论:新仙灵骨葆胶囊具有明显的促进骨折愈合作用,可有效提高骨生物力学强度,抑制炎症反应。OBJECTIVE：To study the effects of new Xianling gubao capsule on fracture healing in rats,and to provide reference for seeking new formula of＂to reduce stockpiles and increase efficiency（to reduce stockpiles and constant efficiency）＂.METHODS：Rats were randomly divided into sham operation group（distilled water）,model group（distilled water）,Xianling gubao capsule group（350 mg/kg）,new Xianling gubao capsule low-dose,medium-dose and high-dose groups（53,105,210 mg/kg）,with 14 rats in each group. Except for sham operation group,right middle femur fracture model was established under the anesthetic state. Rats were given relevant medicine 10 m L/kg intragastrically after woken,once a day,for consecutive 4 weeks.After last administration,body weights of rats were determined;the formation of callus and histopathological changes in fracture were observed;biomechanics of fracture side（fracture stress and bone crushing force）was measured. The levels of inflammatory factors（TNF-α,IL-1 β） in serum were detected by ELISA. RESULTS：Compared with sham operation group,body weight,fracture stress and bone crushing force of fracture side were decreased significantly（P〈0.05 or P〈0.01）,while the serum levels of TNF-α and IL-1 β were increased significantly（P〈0.01）. In model group,the scab was visible around the fracture,and the trabecular bone was not mature and arranged in confusion. Compared with model group,body weights of rats were increased significantly in new Xianling gubao capsule high-dose group and Xianling gubao capsule group（P〈0.05）;fracture stress of

关 键 词：新仙灵骨葆胶囊 大鼠 骨折 骨生物力学 炎症因子 

分 类 号：R285.5[医药卫生—中药学]