僵蚕两种入药形式下的化学成分溶出性能与抗惊厥作用比较  

Comparison of the Dissolution Performance of Chemical Component and Anticonvulsant Effect between Two Application Forms of Bombyx mori

作　　者：程雪娇[1] 胡美变 刘玉杰[1] 肖禾 吴纯洁[1] CHENG Xuejiao;HU Meibian;LIU Yujie;XIAO He;WU Chunjie(College of Pharmacy, Chengdu University of TCM, Chengdu 611137, China;Chengdu Minjiang Source Pharmaceutical Co., Ltd., Chengdu 611000, China)

机构地区：[1]成都中医药大学药学院,成都611137 [2]成都岷江源药业股份有限公司,成都611000

出　　处：《中国药房》2018年第9期1242-1245,共4页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81773906);国家中药标准化项目(No.ZYBIH-Y-SC-41)

摘　　要：目的:对僵蚕煎液和粉末冲服两种入药形式下化学成分溶出性能与抗惊厥作用的差异性进行研究,为僵蚕入药方式的选择提供参考。方法:测定僵蚕煎液和粉末仿生胃液的干浸膏得率,采用高效液相色谱法测定僵蚕煎液和粉末仿生胃液中草酸铵的含量,采用二喹啉甲酸法测定僵蚕煎液和粉末仿生胃液中蛋白质含量。取小鼠按随机数字表法分为正常组(1%羧甲基纤维素钠溶液)、模型组(1%羧甲基纤维素钠溶液)、阳性组(苯妥英钠,2 mg/kg)以及僵蚕煎液和粉末混悬液的高、中、低剂量组(以生药计分别为0.75、1.5、3 g/kg),每组20只;灌胃给药60 min后给予小鼠电刺激,记录小鼠惊厥率。结果:僵蚕煎液与粉末胃液溶液的干浸膏得率分别为22.08%、26.40%(P<0.05),草酸铵含量分别为11.22%、16.83%(P<0.05),蛋白质含量分别为3.39%、4.92%(P<0.01)。与正常组比较,模型组小鼠惊厥率显著升高(P<0.01);与模型组比较,各给药组小鼠的惊厥率均显著降低(P<0.05或P<0.01),且僵蚕粉末混悬液组小鼠惊厥率均低于同剂量煎液组。结论:僵蚕粉末仿生胃液中化学成分的溶出性能优于煎液,且僵蚕粉末抗惊厥效果较煎液好。OBJECTIVE：To study the difference of dissolution performance of chemical component and anticonvulsant effect of Bombyx mori decoction and powder taken with water,and to provide reference for the selection of application forms of B. mori.METHODS：The yield of dry extract was determined for decoction and powder biomimetic gastric juice of B. mori. HPLC method was used to detect the content of ammonium oxalate in decoction and powder biomimetic gastric juice of B. mori. The content of protein in decoction and powder biomimetic gastric juice of B. mori was determined with bicinchoninic acid（BCA）method. Mice was divided into normal group（1% Sodium carboxymethylcellulose solution）,model group（1% Sodium carboxymethylcellulose solution）,positive group（phenytoin,2 mg/kg） and B. mori decoction and powder suspensions high-dose,medium-dose and low-dose groups（0.75,1.5,3 g/kg by crude drug）according to random number table,with 20 mice in each group. After 60 min of intragastric administration,electric stimulation was conducted,and the rate of convulsion in mice was recorded. RESULTS：The yields of dry extract were 22.08% and 26.40% in decoction and powder gastric juice of B. mori（P〈0.05）;the contents of ammonium oxalate were 11.22% and 16.83%（P〈0.05）, and the contents of protein was 3.39% and 4.92%（P〈0.01）.Compared with normal group,convulsion rate of mice was increased significantly in model group（P〈0.01）;compared with model group,convulsion rates of mice were decreased significantly in administration groups（P〈0.05 or P〈0.01）,and the convulsion rate of mice in B. mori powder suspensions group was lower than decoction group. CONCLUSIONS：The dissolution performance of the chemical component from gastric juice of B. mori powder is better than that of decoction, and the anticonvulsant effect of B. mori powder is better than decoction.

关 键 词：僵蚕 粉末 煎液 干浸膏得率 草酸铵 蛋白质 

分 类 号：R284.2[医药卫生—中药学]