无梗五加果实3种提取物对人肝癌细胞SMMC-7721增殖和凋亡的影响  

Effects of 3 Extracts of Acanthopanax sessiliflorus Fruits on the Proliferation and Apoptosis of Human Hepatocarcinoma Cells SMMC-7721

作　　者：刘玉强[1] 管美玉 孙建之 才谦[1] LIU Yuqiang;GUAN Meiyu;SUN Jiaxlzhi;CAI Qiaxl(College of Pharmacy, Liaoning University of TCM, Liaoning Dalian 116600, China)

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600

出　　处：《中国药房》2018年第9期1252-1255,共4页China Pharmacy

摘　　要：目的:研究无梗五加果实3种提取物对人肝癌细胞SMMC-7721增殖、细胞周期和凋亡的影响,为明确其抗肿瘤作用机制提供参考。方法:采用MTT法考察低、中、高质量浓度的无梗五加果实乙醇提取物(0.92、1.84、3.68 mg/m L)、粗多糖提取物(0.06、0.12、0.24 mg/m L)和精多糖提取物(0.04、0.08、0.16 mg/m L)分别作用24、36、48 h后对人肝癌细胞SMMC-7721的增殖抑制作用;采用流式细胞术考察1.84 mg/m L乙醇提取物、0.24 mg/m L粗多糖提取物和0.16 mg/m L精多糖提取物分别作用24 h后对人肝癌细胞SMMC-7721细胞周期和细胞凋亡的影响。以上试验均设阴性对照(只加细胞不加药物)。结果:与阴性对照比较,无梗五加果实3种提取物均可显著抑制人肝癌细胞SMMC-7721的增殖(P<0.01),显著降低G0/G1、G2/M期细胞的百分率(P<0.01),显著升高S期细胞的百分率(P<0.01),显著升高细胞凋亡率(P<0.05),其中以无梗五加果实乙醇提取物的作用最为明显。结论:无梗五加果实3种提取物均能抑制人肝癌细胞SMMC-7721的增殖,阻滞其细胞周期于S期,诱导其凋亡。OBJECTIVE：To study the effects of 3 extracts of Acanthopanax sessiliflorus fruits on the proliferation and apoptosis of human hepatocarcinoma cells SMMC-7721,and to provide reference for confirming the mechanism of anti-tumor effect. METHODS：MTT assay was adopted to investigate the effects of low-mass concentration,medium-mass concentration and high-mass concentration of ethanol extract（0.92,1.84,3.68 mg/m L）,crude polysaccharide extract（0.06,0.12,0.24 mg/m L）and refined polysaccharide extract（0.04, 0.08, 0.16 mg/m L） from A. sessiliflorus fruits on the proliferation and apoptosis of SMMC-7721 cells after treated for 24,36,48 h,respectively. Flow cytometry was used to investigate the effects of 1.84 mg/m L ethanol extract,0.24 mg/m L crude polysaccharide extract and 0.16 mg/m L refined polysaccharide extract on cell cycle and cellapoptosis after treated for 24 h. The above tests were all negative control（only adding cells without drugs）. RESULTS：Compared with negative control,3 extracts of A. sessiliflorus fruits could significantly inhibit the proliferation of SMMC-7721 cells（P〈0.01）,could significantly decrease the percentage of SMMC-7721 cells in G0/G1 and G2/M phase（P〈0.01）,could significantly increase the percentage of SMMC-7721 cells in S phase（P〈0.01） and the apoptosis rate of SMMC-7721 cells（P〈0.05）;especially the effects of ethanol extract from A. sessiliflorus fruits were the most obvious. CONCLUSIONS：Three extracts of A.sessiliflorus fruits can inhibit the proliferation of human hepatocarcinoma SMMC-7721 cells,block SMMC-7721 cells in S phase and induce the apoptosis of SMMC-7721 cells.

关 键 词：无梗五加果实 乙醇提取物 粗多糖提取物 精多糖提取物 人肝癌细胞SMMC-7721 增殖 凋亡 

分 类 号：R285.5[医药卫生—中药学]