新型人腺病毒55型在中国10年的持续流行及其基因进化分析  

Continued circulation and phylogenetic analysis of human adenovirus-55 in China during 2006-2016

作　　者：毛乃颖[1] 朱贞[1] 雷振强 李岩[2] 黄芳[3] 尹洁[4] 陈萌[3] 向星宇[5] 李红 唐浏英[1] 崔爱利[1] 李忠[6] 刘倜[6] 许文波[1] Mao Naiying;Zhu Zhen;Lei Zhenqiang;Li Yan;Hong;Tang Liuying;Cui Aili;Li Zhong;Liu Ti;Huang Fang;Yin Jie;Chen Meng;Xiang Xingyu;Li Xu Wenbo(National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;Hebei Provincial Center for Disease Control and Prevention, Shijiazhuang 050021, China;Belting Center for Disease Control and Prevention, Beijing 100013, China;Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, Chin;Hunan Provincial Center for Disease Control and Prevention, Changsha 410005, China;Shandong Provincial Center for Disease Control and Prevention, Jinan 250014, Chin)

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京102206 [2]河北省疾病预防控制中心,石家庄050021 [3]北京市疾病预防控制中心,100013 [4]云南省疾病预防控制中心,昆明650022 [5]湖南省疾病预防控制中心,长沙410005 [6]山东省疾病预防控制中心,济南250014

出　　处：《中华实验和临床病毒学杂志》2018年第2期124-129,共6页Chinese Journal of Experimental and Clinical Virology

基　　金：十二五国家科技重大专项（2013ZX10004-202,2012ZX10004-201-003）;国际合作课题（WPCHN1409379）;国家重点研发计划（2016YFC1200905）

摘　　要：目的了解新型腺病毒55型（HAdV-55）在中国的流行状况和基因变异及进化规律，为其预防和控制提供科学依据。方法本研究对2011—2014年在中国5个省市（北京、河北、山东、湖南和云南）发热呼吸道症候群患者标本中分离到的HAdV-55毒株进行了hexon、fiber和penton base三个基因的序列测定，同时下载GenBank数据库中中国以及全球其他国家和地区、不同时间流行的HAdV-55病毒株的三个基因的序列进行比对，并利用多种序列分析软件进行了同源性和基因进化分析。结果2006—2016年10年期间，在中国的15个省（市、自治区和直辖市）分离到HAdV-55病毒。进化分析结果显示，HAdV-55的三个主要结构基因均高度保守，其核苷酸和氨基酸序列同源性均大于99%；HAdV-55病毒在进化上是极为保守稳定，在进化树上聚集在一个分支，并有一定的时间聚集进化趋势。HAdV-55的hexon和fiber基因进化速率分为5.228×10^－5和1.238×10^－4substitutions/site/year，hexon基因的最早共同进化祖先tMRCA可追溯到1963年。结论HAdV-55已经在中国形成了广泛传播和持续流行态势，建立有效的监测系统和开展疫苗相关研究对于其预防和控制至关重要。Objective To better understand the evolution and epidemiology of human adenovirus- 55 （HAdV-55） and provide a scientific basis for the prevention and control of the epidemic of HAdV-55 in China. Methods HAdV-55 isolates from 5 provinces in China included Beijing, Hebei, Shandong, Hunan and Yunnan were collected during 2011-2014. The hexon, fiber and penton base gene were sequenced, and compared with other strains of HAdV-55 sequences downloaded from GenBank for homology and evolutionanalysis. Results During the past decade, HAdV-55 was found in 15 provinces throughout China. Genetic and phylogenetic analysis showed that the HAdV-55 virus is highly conservative in evolution due to aggregation in a branch in the evolutionary tree. However, bayesian phylogenetic tree shows a certain time evolution trend. The evolution rate of hexon and fiber gene of HAdV-55 are 5. 228 x 10 -Sand 1. 238 x 10 -4 substitutions/site/year respectively, and the latest coevolutionary ancestor tMRCA of hexon gene can be traced back to 1963. Conclusions HAdV-55 has been widely spread and continued circulating in China. Establishing effective monitoring system and conducting vaccine related research is very important for its control and prevention.

关 键 词：新型腺病毒55型 急性呼吸道感染 贝叶斯进化分析 基因 

分 类 号：R181.3[医药卫生—流行病学] R511.8[医药卫生—公共卫生与预防医学]