台州市本地感染登革病毒分子分型及溯源分析  

Genotyping and molecular tracing of the first local infection of dengue virus in Taizhou

作　　者：王东红 裘丹红[1] 翁坚[1] 盛莹[1] 林海江[1] 林春萍[2] 孔超[2] 周静晓 沈伟伟[1] Wang Donghong;Qiu Danhong;Weng Jian;Sheng Ying;Lin Haifiang;Lin Chunping;Kong Chao;Zhou Xiaojing;Shen Weiwei(Taizhou Center for Disease Control and Prevention, Taizhou 318000, China;Huangyan District Center for Disease Control and Prevention, Taizhou 318020, China)

机构地区：[1]台州市疾病预防控制中心,318000 [2]台州市黄岩区疾病预防控制中心,318020

出　　处：《中华实验和临床病毒学杂志》2018年第2期169-172,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的对2016年台州市本地暴发的登革热病例进行实验室检测与溯源分析。方法采集3例患者急性期血清进行抗原、抗体和核酸检测，并用C6/36细胞分离培养登革病毒。用RT-PCR方法扩增全长E基因，测定序列并绘制系统进化树。结果从患者血清中检测到登革I型病毒核酸、IgM抗体和NS1抗原。成功分离到3株I型登革病毒株，这3株病毒在同一进化分支上，属于I型登革病毒中的Genotype I亚型，核苷酸同源性均为99.87%，氨基酸同源性为99.6%～99.8%。结论浙江省台州市本次登革热本地暴发病例可能由广东或东南亚输入而来。Objective To determine the first dengue fever case in Taizhou and trace probable transmission sources. Methods Collected serum of three patients for antigen, antibody and nucleic acid detection. Dengue viruses were isolated and cultured in C6/36 cell. The whole length of E gene was amplified by reverse transcriptase-polymerase chain reaction （RT-PCR） and then sequenced. The phylogenetic tree was drawn. Results Three cases were positive in nucleic acid detection. Two cases were IgM positive. One case was NSI antigen postive. Three strains of type I dengue virus were isolated from samples. The phylogenetic trees shown that the three strains were on the same branch. The identities of nucleotide were 99.87%. The identities of amino acid were 99.6% -99.8%. Conclusions The dengue virus strains isolated in Taizhou was imported from Guangdong or Southeast Asia and caused location infection.

关 键 词：登革病毒 E基因 序列分析 

分 类 号：R440[医药卫生—诊断学] R512.8[医药卫生—临床医学]