高效液相色谱法测定苜蓿中脱落酸的含量  

Determination of abscisic acid in medicago sativa cv. by high performance liquid chromatography

作　　者：王尉[1] 乐胜锋[1] 贺天雨 杜宁[1] 林楠[1] Wang Wei;Le Shengfeng;He Tianyu;Du Ning;Lin Nan(Beijing Center for Physical and Chemical Analysis, Beijing Key Laboratory of Organic Material Detection Technology and Quality Evaluation, Beijing Academy of Science and Technology, Key Laboratory of Analysis and Testing Technology, Beijing 100094, China)

机构地区：[1]北京市理化分析测试中心有机材料检测技术与质量评价北京市重点实验室北京市科学技术研究院分析测试技术重点实验室,北京100094

出　　处：《分析仪器》2018年第2期53-57,共5页Analytical Instrumentation

基　　金：北京市财政资金改革与发展课题(2018ZL0101)

摘　　要：通过对脱落酸(ABA)前处理方法进行优化,并采用高效液相色谱法测定了苜蓿根系样品中ABA的含量。首先,分别对提取溶剂、提取时间、料液比、提取次数进行条件优化,并建立了交联聚乙烯吡咯烷酮(PVPP)和SPE联用的净化方法。结果显示,最佳的前处理方法为提取溶剂80%甲醇,提取时间16h,料液比为1∶20g/mL,提取2次。通过方法学考察,ABA在0.05~1mg/L范围内线性关系良好,检出限为0.02μg/g,定量限为0.04μg/g。该方法稳定性、精密度、重现性良好,加标回收率为91.7~105.2%,可以应用于植物中ABA含量的测定。The optimal extraction conditions of ABA were explored,and the content of ABA in the root of Medicago sativacv.was determined by high performance liquid chromatography.Effects of extraction reagent and extract time,material-liquid ratio and extraction times on extraction yield of ABA were investigated,and a purification method based on the combination of PVPP and SPE was established.The results showed that the optimal extraction conditions were as follows：extraction time 16 h;material-liquid ratio 1：20 g/mL;twice extraction with 80% methanol.The method was of good stability,precision and reproducibility,and the recoveries of ABA in this experiment were in the range of 91.7-105.2%.It can be applied to the determination of ABA content in plants.

关 键 词：脱落酸 高效液相色谱 苜蓿 含量测定 

分 类 号：O657.72[理学—分析化学] S541[理学—化学]