Mg-Sr合金对骨髓间充质干细胞增殖及成软骨分化的研究  

Study on Mg-Sr Alloy for Proliferation and Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells

作　　者：王常成 周子皓 郝瑞胡 田丰德[1] 安宁[1] 肖鹏 谭丽丽[2] 杨柯[2] 郭林[1] WANG Chang-cheng;ZHOU Zi-hao;HAO Rui-hu;TIAN Fens-de;AN Ning;XIAO Peng;TAN Li-li;YANG Ke(Orthopaedics Department, Dalian University Affilicted Zhongshan Hospital, Dalian Liaoning 116001;China Academy of Sciences Metal Research Institute, Shenyang Liaoning 110016)

机构地区：[1]大连大学附属中山医院骨二科,辽宁大连116001 [2]中国科学院金属研究所,辽宁沈阳110016

出　　处：《世界最新医学信息文摘》2018年第2期3-4,14,共3页World Latest Medicine Information Electronic Version

摘　　要：目的探讨Mg-Sr合金对兔骨髓间充质干细胞(BMSCs)的增殖情况以及对成软骨分化的影响。方法抽取兔BMSCs培养、传代,观察细胞生长情况;流式细胞仪检测所抽取细胞表面抗原表达情况及研究成软骨诱导分化情况。实验分为:普通培养基(A组)、纯Mg浸提液(B组)、Mg-Sr合金浸提液(C组)三组,其中A组、B组为对照组;检测Mg、Mg-Sr合金是否对兔BMSCs有细胞毒性,同时观察增殖活性。并对三组兔BMSCs行成软骨诱导分化;在第7、14、21 d,Westen blot检测成软骨分化后Col2A1蛋白表达情况。结果 (1)骨髓细胞生长1-2 d后,呈对数生长;流式细胞仪检测骨髓细胞表面抗原表达CD34(-)、CD45(-)、CD44(+)、CD90(+)、CD106(+);骨髓细胞可成软骨诱导分化。证明为兔BMSCs。(2)Mg、Mg-Sr合金相对增殖率≥1,证明Mg、Mg-Sr合金无细胞毒性。增殖活性:B组及C组对BMSCs的增殖活性均高于A组(P<0.05),B组与C组两组对BMSCs的增殖活性无明显差异(P>0.05)。诱导成软骨后westen blot检测:7、14、21 d均显示B组及C组诱导成软骨后Col2A1蛋白表达高于A组(P<0.05),B组与C组两组诱导成软骨后Col2A1蛋白表达无明显差异(P>0.05)。结论 Mg、Mg-Sr合金无细胞毒性;Mg-Sr合金及纯Mg对兔BMSCs的增殖及成软骨分化均有促进作用。Mg-Sr合金与纯Mg对兔BMSCs的增殖、成软骨分化的影响无不同。Objective to investigate effect of Mg-Sr alloy on proliferation and chondrogenic differentiation of rabbit bone marrow mesenchymal stem cells（BMSCs）. Methods choose rabbit BMSCs for culture and generation, observe cell growth. Detect cell surface antigen expression with flow cytometry, and study chondrocytes differentiation. Experiment had three groups： normal culture medium（group A）, pure Mg leaching solution（group B） and Mg-Sr alloy leaching solution（group C）. Group A and B were control group, detect if Mg, Mg-Sr alloy has cytotoxicity for rabbit BMSCs, and observe proliferative activity. Carry on chondrogenic differentiation for three groups of rabbit BMSCs, detect Col2 A1 protein expression after chondrogenic differentiation with Westen blot on the 7, 14, 21 d. Results 1. after 1-2 d growing, bone marrow cell showed logarithmic growth; detect bone marrow cell surface antigen expression with flow cytometry： CD34（-）, CD45（-）, CD44（＋）, CD90（＋）, CD106（＋）, chondrogenic differentiation of bone marrow cells, showed it was rabbit BMSCs. 2. relative proliferation rate of Mg, Mg-Sr alloy≥1, showed Mg, Mg-Sr alloy had no cytotoxicity. Proliferation activity of BMSCs of group B and C was higher than group A,（P〈0.05）, proliferation activity of BMSCs of Group B and C showed no significant difference,（P〈0.05）. After induction of chondrogenesis, westen blot detection on 7, 14 and 21 d showed, Col2 A1 protein expression after chondrogenesis induction of group B and C was higher than group A,（P〈0.05）, Col2 A1 protein expression showed no significant difference after chondrogenesis induction of group B and C. Conclusion Mg and Mg-Sr alloy has no cytotoxicity, Mg-Sr alloy and pure Mg has promoting effect for proliferation and chondrogenic differentiation of rabbit BMSCs. Mg-Sr alloy and pure Mg has no different effect on proliferation and chondrogenic differentiation of rabbit BMSCs.

关 键 词：Mg-Sr合金 骨髓间充质干细胞 增殖活性 成软骨分化 

分 类 号：R3[医药卫生—基础医学]