解淀粉芽孢杆菌TWC2诱导甘蔗抗病性相关防御酶系的研究  

The Induction of Defense-related Enzymes in Sugarcane by Bacillus amyloliquefaciens TWC2

作　　者：梁艳琼[1] 吴伟怀[1,2] 黄兴 习金根[1] 李锐[1] 郑金龙[1] 贺春萍[1] 易克贤[1] LIANG Yan-qiong;WU Wei-huai;HUANG Xing;XI Jin-gen;LI Rui;ZHENG Jin-long;et al(Environment and Plant Protection Institute, CATAS / Key Laboratory for Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests, Ministry of Agriculture / Hainan Key Laboratory for Detection and Control of Tropical Agricultural Pests, Haikou 571101, Hainan;Opening Project Fund of Key Laboratory of Rubber Biology and Genetic Resource Utilization, Ministry of Agriculture / State Key Laboratory Breeding Base of Cultivation ＆ Physiology for Tropical Crops / Danzhou Investigation ＆ Experiment Station of Tropical Crops, Ministry of Agriculture, Danzhou 571737, Hainan)

机构地区：[1]中国热带农业科学院环境与植物保护研究所/农业部热带农林有害生物入侵检测与控制重点开放实验室/海南省热带农业有害生物检测监控重点实验室,海南海口571101 [2]农业部橡胶树生物学与遗传资源利用重点实验室/省部共建国家重点实验室培育基地-海南省热带作物栽培生理学重点实验室/农业部儋州热带作物科学观测实验站,海南儋州571737

出　　处：《中国糖料》2018年第3期13-16,共4页Sugar Crops of China

基　　金：中央级公益性科研院所基本科研业务费专项(NO.2015hzs1J014;NO.2012hzs1J012;NO.2014hzs1J012);中国热带农业科学院橡胶研究所省部重点实验室科学观测实验站开放课题(RRI-KLOF201506)

摘　　要：为了探讨解淀粉芽孢杆菌TWC2诱导甘蔗抗病性机理,开发有效的生防制剂,采用比色法测定了解淀粉芽孢杆菌TWC2诱导甘蔗叶片后对叶片中多酚氧化酶(PPO)、过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)、超氧化物岐化酶(SOD)、过氧化氢酶(CAT)5种防御酶活性及丙二醛(MDA)含量的影响。结果表明,解淀粉芽孢杆菌TWC2处理后的甘蔗叶片CAT、POD、SOD、PAL、PPO比未处理的显著提高;MDA含量比未处理的显著降低;且经10倍稀释液处理的甘蔗叶片中防御酶活性最高,诱导植株抗病性效果最佳。喷施TWC2稀释液可促使甘蔗抗病相关酶活性升高,诱导甘蔗植株产生系统抗性。诱导抗性可能是TWC2防治甘蔗病害的重要机制之一。To probe into the biocontrol mechanism of Bacillus amyloliquefaciens TWC2 to induce sugarcane disease resistance and develop effective biocontrol agents, we determined the activities of polyphenol oxidase（PPO）, peroxidase（POD）, superoxide dismutase（SOD）, catalase（CAT）, phenylalanine（PAL） and content of maleic dialdehye（MDA） of sugarcane after being inoculated with TWC2. The results showed that the activities of POD,PPO, SOD, PAL and CAT in sugarcane leaves increased significantly after the treatment of fermented liquid,while the level of MDA was significantly lower than that of the untreated ones. The highest activity of defensive enzyme was found in sugarcane leaves,which was treated with 10 times diluent of TWC2, and the effect of inducing disease resistance of sugarcane was the best. Spraying fermentation liquor of TWC2 can increase activities of resistance-related enzymes, to induce systemic resistance of sugarcane plants. Further-more, the induced disease resistance may be an important mechanism for using B. amyloliquefaciens to control fungal diseases of sugarcane.

关 键 词：甘蔗 解淀粉芽孢杆菌 诱导抗病性 防御酶 

分 类 号：S566.1[农业科学—作物学]