Fe_3O_4@PEG磁性纳米粒子的合成及其对阿霉素的载药性能  

Magnetic Fe_3O_4@PEG nanoparticles synthesis and application for doxorubicin delivery

作　　者：张坤[1] 江峰[1] 季帆 谷元亮 苏红[2] 赵进顺[2] 张剑锋[1] ZHANG Kun;JIANG Feng;JI Fan;GU Yuan-liang;SU Hong;ZHAO Jin-shun;ZHANG Jian-feng(Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China;Medical School, Ningbo University, Ningbo 315211, China)

机构地区：[1]宁波大学材料科学与化学工程学院,浙江宁波315211 [2]宁波大学医学院,浙江宁波315211

出　　处：《宁波大学学报（理工版）》2018年第3期60-64,共5页Journal of Ningbo University：Natural Science and Engineering Edition

基　　金：浙江省公益性技术应用研究计划(2015C31044);宁波市自然科学基金(2014A610113)

摘　　要：将通过化学共沉淀方法得到的Fe_3O_4纳米粒子依次用柠檬酸和双羧基化的聚乙二醇进行修饰,合成了具有磁靶向的纳米粒子载体(Fe_3O_4@PEG),分别用X射线衍射、红外光谱、扫描电镜、粒径分布、热重分析对其结构进行了表征,将合成的磁性纳米粒子负载上模型药物阿霉素(DOX),得到新的载药体系Fe_3O_4@PEG-DOX,研究了该载药体系的载药特征和释放行为.研究发现,该载体在水中载药率为85%,累积释放实验表明,在72 h内,pH 5.0时释放率达到71%,远高于pH 7.4时43%的释放率,体外细胞实验表明负载阿霉素的磁性纳米载体的抗肿瘤活性没有损失,与未经修饰的阿霉素的抗肿瘤活性相当.研究结果表明,通过柠檬酸连接的Fe_3O_4@PEG纳米粒子对阿霉素具有较好的修饰作用,是一种较为理想的阿霉素载药系统.Magnetic Fe3O4@PEG nanoparticles were prepared by modification of Fe3O4 with citric acid and di-carboxylic polyethylene glycol（PEG）. The structure of magnetic nanoparticles was characterized by XRD, FT-IR, SEM, PSD, TGA. Doxorubicin（DOX） was loaded by the magnetic Fe3O4@PEG nanoparticle to establish a novel drug delivery system（DDS）, Fe3O4@PEG-DOX. The loading capacity of nano-carrier and the release behavior of Fe3O4@PEG-DOX was studied. The results reveal that the loading capacity of the nano-carrier is 85%. The cumulative release test reveals that the drug release rate reaches to 71% in pH 5.0 PBS buffer by contrast a lower drug release rate of 43% in pH 7.4 with 72 h. In vitro experiments indicate that Fe3O4@PEGDOX shows the same antitumor activity as the unmodified doxorubicin without significant loss of cytotoxicity. The research forecasts that citric acid coated Fe3O4@PEG nanoparticles is a prospective DDR system for doxorubicin.

关 键 词：阿霉素 纳米粒子 四氧化三铁 药物修饰 聚乙二醇化 

分 类 号：TQ460.1[化学工程—制药化工]