不同激素浓度组合对甜叶菊幼叶诱导愈伤和芽分化的影响  

Effect of Different Hormone Combinations on Callus Initiation and Shooting of Stevia rebaudiana Bertoni

作　　者：苏彩霞[1] 李惠芝[1] 王玉泉[1] 宝牡丹 赵文博[1] SU Cai-xia;LI Hui-zhi;WANG Yu-quan;BAO Mu-dan;ZHAO Wen-bo(Department of Agriculture, Animal Hushandry and Bioengineering, XingAn Vocational and Technical College, Ulanhot 137400, China)

机构地区：[1]兴安职业技术学院农牧与生物工程系,内蒙古乌兰浩特137400

出　　处：《黑龙江农业科学》2018年第4期9-12,共4页Heilongjiang Agricultural Sciences

基　　金：2015年内蒙古兴安盟科技处重点资助项目

摘　　要：为提高甜叶菊的组织培养效率,以甜叶菊幼叶为外植体,研究75%消毒酒精和0.1%氯化汞的不同消毒时间组合对外植体的消毒效果;以及不同激素浓度组合对外植体愈伤组织的诱导和芽分化的影响。结果表明:甜叶菊幼叶外植体材料的最佳灭菌条件为75%消毒酒精30s,0.1%氯化汞2min;最佳诱导愈伤组织和分化芽培养基为MS+1.5mg·L^(-1) 6-BA+0.5mg·L^(-1) NAA,此时不定芽增长率为85.7%,外植体适宜的生根培养基为1/2 MS十0.2mg·L^(-1)IAA,生根率为88.6%。In order to improve the culture efficiency of Stevia rebaudiana Bertoni,taking the young leaves as materials to study the effect different disinfection time of 75% disinfected alcohol and 0.1% mercuric chloride,and different hormone combinations on callus initiation and shooting.The results showed that the optimum sterilization conditions for the leaves of Stevia rebaudiana were 75% sterile alcohol 30 sand 0.1% mercuric chloride for 2 min.The best shooting medium was MS＋1.5 mg·L（-1） 6-BA＋0.5 mg·L（-1） NAA,and the ratio of inducing was 85.7%.The optimum rooting medium was 1/2 MS＋0.2 mg·L（-1） IAA,and the ratio of rooting was 88.6%.

关 键 词：激素浓度组合 甜叶菊 诱导愈伤和分化芽 

分 类 号：S566.9[农业科学—作物学]