新疆绵羊肺炎克雷伯菌分离鉴定及部分生物学特性研究  

Isolation and Identification of Sheep Klebsiella pneumoniae in Xinjiang and the Research of Its Partial Biological Characteristics

作　　者：赵洁雅[1] 陈世军[2] 杨会国[3] 郝耿[3] 汪萍[2] 马文戈[2] 夏俊[2] ZHAO Jie-ya;CHEN Shi-jun;YANG Hui-guo;HAO Geng;WANG Ping;MA Wen-ge;XIA Jun(Xinjiang Agriculture University, Urumqi 830052, China;Veterinary Research Institute, Xinjiang Academy of Animal Science, Animal Clinical Medicine Research Center, Urumqi 830052,China;Institute of Animal Science, Xinjiang Academy of Animal Science,Urumqi 830052, China)

机构地区：[1]新疆农业大学,新疆乌鲁木齐830052 [2]新疆畜牧科学院兽医研究所/动物临床医学研究中心,新疆乌鲁木齐830052 [3]新疆畜牧科学院畜牧研究所,新疆乌鲁木齐830052

出　　处：《黑龙江农业科学》2018年第4期57-60,共4页Heilongjiang Agricultural Sciences

基　　金：边境地区家养动物外来动物疫病监测溯源技术研究资助项目(2017YFD0501801);国家绒毛用羊产业技术体系寄生虫病防治岗位资助项目(CARS-39-15);肉羊产业提质增效关键技术研究与示范资助项目(2017B01005-2)

摘　　要：为促进绵羊健康养殖生产和对肺炎克雷伯氏菌感染的诊断和防治,采集病死绵羊的脏器组织并从中分离出1株革兰阴性短杆菌,对细菌进行鉴定、药敏试验、毒力试验、16S rRNA保守区的克隆、测序和序列比对。结果表明:PCR条带长度约为1 500bp,基因序列与肺炎克雷伯氏菌同源性高达99%,最终确诊为肺炎克雷伯菌。动物试验及药敏试验表明该菌具有较强的致病性,对头孢他啶等敏感,对青霉素耐药,推断此株肺炎克雷伯氏菌为导致绵羊死亡的主要病原菌。In order to promote the healthy production of sheep and provide reference for the diagnosis and prevention of Klebsiella pneumoniae infection,the organ tissues of dead sheep were collected,and a gram-negative bacilli was isolated from it.The bacteria were identified and the drug sensitivity test,virulence test,16 S rRNA conserved region cloning,sequencing and sequence alignment were carried out.The results showed that the length of PCR band was about 1 500 bp,and the homology of Klebsiella pneumoniae was up to 99%,and Klebsiella pneumoniae was finally confirmed.Animal test and drug sensitivity test showed that the bacteria had strong pathogenicity,and the bacteria was sensitive to ceftazidime and was resistant to penicillin.It was inferred that Klebsiella pneumoniae was the main pathogen causing the death of sheep.

关 键 词：绵羊 肺炎克雷伯氏菌 分离鉴定 

分 类 号：S852.61[农业科学—基础兽医学]