高效液相色谱法测定人血浆中芬太尼浓度  

Determination of fentanyl in human plasma by HPLC

作　　者：杨明 季方茹[1] 雷力力[1] 张欣然 方洪壮 陈佳慧 YANG Ming,JI Fang-ru,LEI Li -li, ZHANG Xin- ran, FANG Hong -zhuang, CNEN Jia -hui(The First Affiliated Hospital of Jialnusi University, Jialnusi 154003 ,Chin)

机构地区：[1]佳木斯大学附属第一医院,黑龙江佳木斯154003

出　　处：《黑龙江医药科学》2018年第2期38-40,共3页Heilongjiang Medicine and Pharmacy

基　　金：佳木斯大学研究生科技创新项目;编号:YM2016_110

摘　　要：目的:建立测定人血浆中芬太尼浓度的高效液相色谱法。方法:本实验采用内标法,舒芬太尼为内标物。以正己烷-无水乙醇(95:5)为提取溶剂;以采用Sinochrom ODS-BP(4.6mm×200mm,5μm)色谱柱,甲醇-乙腈-0.13mol·L^(-1)%乙酸铵水溶液(45:24:31)为流动相(pH=7.5),流速为1.0 mL·min-1,柱温为室温,检测波长为215nm。结果:芬太尼的血浆浓度在31.25 ng·mL^(-1)~1000 ng·mL^(-1)范围内线性关系良好(r=0.9996),最低定量限为31.25ng·mL^(-1),方法回收率为94.2%~102.3%,萃取回收率70.5%~77.1%,日内、日间精密度均小于15%。结论:本方法操作简单、准确度和灵敏度高,适用于临床芬太尼血药浓度检测及药动学的研究。Objective： To determine the concentration of fentanyl in human plasma by HPLC. Method： Internal standard method was used in this experiment. Sufentanil was the internal standard and n-hexane-absolute ethanol（95：5） was extraction solvent. The chromatographic separation was carried out on Sinochrom ODS-BP（4. 6 mm × 200 mm,5μm） column. The mobile phase consisted of solvent Methanol-acetonitrile-0. 13 mol·L-1% aqueous ammonium acetate solution（45：24：31,pH = 7. 5）. The flow rate was 1. 0 mL·min-1; the column temperature was set to room temperature and the UV detection wavelength was 215 nm. Results： The calibration curve for fentanyl was linear in the range of 31. 25 ng·mL-1~ 1000 ng·mL-1（r = 0. 9996）. The lowest quantification limit was 31. 25 ng·mL-1; the methodological recovery was 94. 2% ~ 102. 3% and the extraction recovery was 70. 5% ~ 77. 1%. Both the intra-day and inter-day RSDs were less than 15%. Conclusion： The method is simple,accurate and sensitive,and is suitable for the determination of Fentanyl concentration in plasma clinically and pharmacokinetics study.

关 键 词：芬太尼 高效液相色谱法 血浆浓度 

分 类 号：R614[医药卫生—麻醉学]