蛋氨酸铬对鲤糖代谢相关酶活性及IR、GLUT2和SGLT基因表达的影响  

Effects of chromium methionine on activities of glyco-metabolism-related enzymes and expression of IR,GLUT2 and SGLT genes in common carp Cyprinus carpio

作　　者：崔培 尹帅 孙金辉 程镇燕 白东清 乔秀亭 张宝龙 翟胜利 CUI Pei;YIN Shuai;SUN Jln-hui;CHENG Zhen-yan;BAI Dong-qing;QIAO Xiu-ting;ZHANG Bao-long;ZHAI Sheng-li(College of Fisheries Science, Tianjin Key LaboratoD- of Aqua-ecology and Aquaculture, Tianjin Agricultural Lniversity, Tianjin 300384, China;Tianjin Chenhui Feed Company Limited, Tianjin 301800, China)

机构地区：[1]天津农学院水产学院天津市水产生态与养殖重点实验室,天津300384 [2]天津晨辉饲料有限公司,天津301800

出　　处：《大连海洋大学学报》2018年第3期316-322,共7页Journal of Dalian Ocean University

基　　金：天津市高等学校创新团队项目(TD12-5018);天津市自然科学基金资助项目(14JCQNJC15100);浙江省重中之重学科开放基金资助项目(xkzsc1501);农业部热带亚热带水产资源利用与养殖重点实验室开放基金资助项目

摘　　要：为探究蛋氨酸铬(CrMet)对鲤Cyprinus carpio糖代谢相关酶活性及糖代谢相关基因表达的影响,以酪蛋白为蛋白源,豆油为脂肪源,配制7组纯化饲料,其中Cr^(3+)水平分别为0(对照)、0.1、0.2、0.4、0.8、1.6、3.2 mg/kg。选取初始体质量为(40.95±4.80)g的鲤,随机分为7组,分别投喂7种饲料,每个组设置3个重复,每个重复放60尾鱼,饲养8周后,检测其肝胰脏糖代谢相关酶活性;禁食48 h后再投喂,再投喂0、3、6、12、24、48 h时检测Cr^(3+)水平为0(对照)、0.8、3.2 mg/kg时鱼肝胰脏IR、GLUT2和肠道SGLT基因的表达量。结果表明:添加0.8 mg/kg Cr^(3+)组的组己糖激酶(HK)、丙酮酸激酶(PK)、磷酸果糖激酶(PFK)和琥珀酸脱氢酶(SDH)活力均显著高于对照组(P<0.05),磷酸酵式丙酮酸激酶(PEPCK)活力显著低于对照组(P<0.05);而添加Cr^(3+)并未对葡萄糖-6-磷酸脱氢酶(G6PDH)活力产生显著影响(P>0.05);投喂24、48 h时,0.8 mg/kg Cr^(3+)组IR mRNA表达量显著高于0、3.2mg/kg Cr^(3+)组(P<0.05);投喂3 h时,0.8、3.2 mg/kg Cr^(3+)组GLUT2 mRNA表达量均显著高于对照组(P<0.05),12、24、48 h时,0.8 mg/kg Cr^(3+)组GLUT2 mRNA表达量显著高于0、3.2 mg/kg Cr^(3+)组(P<0.05);而不同Cr^(3+)添加水平对鲤肠道SGLT mRNA表达量无显著性影响(P>0.05)。研究表明,在饲料中添加CrMet能够提高鲤对糖的利用能力,建议Cr^(3+)添加水平为0.8 mg/kg。A feeding trial was conducted to evaluate the effects of dietary chromium methionine （CrMet） on glyeo- metabolism-related enzymes, and expression of IR, GLUT2 and SGLT genes in common carp Cyprinus carpio Com- mon earp with body weight of （40.95±4.80） g were reared in a rearing system consisting of 21 plastic tanks and fed purified diets containing Cr3＋ at a dose of 0（control group） , 0.1, 0.2, 0.4, 0.8, 1.6, and 3.2 mg/kg for 8 weeks when activities of glyeo-metabolism-related enzymes were tested. IR, and GLUT2 mRNA expression levels in hepa- topanereas and SGLT mRNA expression levels in intestine were analyzed in 0.8 mg/kg group and 3.2 mg/kg group during the refeeding alter 48 h lasting. The resuhs showed that the fish fed diets containing 0.8 mg/kg Cr3＋ had sig- nificantly higher activities of hexokinase （ HK）, pyruvate kinase （ PK）, phosphofruetose kinase （ PFK）, and sueei- hate dehydrogenase （SDH） and lower phosphopyruvate kinase （PEPCK） activity than the fish in 0 mg/kg group did（P〈0.05）, without significant differenee in G6PDH activity betwween the control and Cr3＋ supplementation groups（P〉0.05）. There was significantly higher IR mRNA expression level in 0.8 mg/kg Cr3＋ group than that in 0 mg/kg group 24 h and 48 h alter refeeding（ P〈0.05 ） , significantly higher GLUT2 mRNA expression level in 0.8 and 3.2 mg/kg Cr3＋ groups than that in 0 mg/kg group 3 h alter refeeding（P〈0.05）. 12, 24 and 48 h alter refeeding, GLUT2 mRNA expression level was significantly higher in 0.8 mg/kg Cr3＋ group than in 0 and 3.2 mg/kg groups（P〈0.05） , without significant effect on SGLT mRNA expression level in intestine of common earp fed diets containing differert Cr3＋ level. The findings indicate that dietary addition of Cr3＋ leads to significant impact on the carbohydrate utilization of common earp and 0.8 mg/kg is the optimal level of Cr3＋ in the common earp diet under this experimental condition.

关 键 词：蛋氨酸铬 鲤 糖代谢相关酶 IR基因 GLUT2基因 SGLT基因 

分 类 号：S965[农业科学—水产养殖]