斑点叉尾“腹水症”病原的分离鉴定及体外芽孢杆菌拮抗试验  

Isolation and identification of the pathogen Aeromonas sobria from Ictalurus punctatus and the Bacillus antagonistic experiment in vitro

作　　者：王亚军[1] 魏文娟 潘厚军[1] 颜曦[1] 王芳[1] 石存斌[1] WANG Ya-jun;WEI Wen-juan;Pan Hou-jun;YAN Xi;Wang Fang;SHI Cun-bin(Pearl River Fisheries Research Institute, Chbwse Academy of Fishery Science;Key Laboratory of Fishery Drug Development, Ministry of Agricultur;Key Laboratory of Aquatic Animal Immune Technology, Guangzhou 510380, China)

机构地区：[1]中国水产科学研究院珠江水产研究所,农业部渔用药物创制重点实验室,广东省水产动物免疫技术重点实验室,广州510380

出　　处：《淡水渔业》2018年第3期72-77,共6页Freshwater Fisheries

基　　金：农业部948项目(2016-X57);佛山市水生动物疫病监测与防疫检疫专项(0809-1641FSC3A404);广东省科技计划项目(20138090600007);广东省海洋渔业科技与产业发展专项(201500815)

摘　　要：为了解斑点叉尾(Ictalurus punctatus)"腹水症"的发病原因,提高防控技术,试验在佛山市某渔场患典型"腹水症"的病上分离出优势菌落,通过形态学观察、生化及分子生物学鉴定确定该细菌为温和气单胞菌(Aeromonas sobria)。通过对健康斑点叉尾的回归感染实验发现,与原感染鱼发病症状相似,且分离鉴定得到相同病原菌。体外药敏试验结果显示:该温和气单胞菌菌株对恩诺沙星、氟苯尼考等7种药物敏感,而对四环素、强力霉素耐药。本研究还探讨了4种芽孢杆菌对分离的温和气单胞菌的拮抗作用,结果表明地衣芽孢杆菌对该病原的拮抗作用最好。To research on the etiology of the ascites disease pathogens from Ictalurus punctatus and improve the prevention and control technology , the dominant bacterial colonies were isolated from I. punctatus with typical symptoms from a fishing ground in Foshan. The bacteria was identified as Aeromonas sobria through biochemical and molecular biological methods. The healthy I. punctatus were recurrent infected by intraperitoneal injection and showed similar symptoms with those naturally infected individuals. Bacteria separated from the visceral tissue were identified as Aeromonas sobria, indicating it cause the infection. The drug sensitivity test in vitro results showed that A. sobria was sensitive to enrofloxacin, floffenicol and other 7 antibiotics, but it indicated drug resistance to acheomycin and doxycycline. This study investigated the antago- nism of isolated A. sobria to 4 species of bacillus, and the results showed that Bacillus licheniformis had the strongest antag- onistic effect against the pathogen.

关 键 词：斑点叉尾(Ictalurus punctatus) 腹水症 温和气单胞菌(Aeromonas sobria) 病原鉴定 药敏试验 芽孢杆菌拮抗试验 

分 类 号：S941.42[农业科学—水产养殖]