鸭源鸡杆菌flfA基因的克隆及功能区域分析  

Cloning of the flfA Gene of Gallibacterium anatis and Analysis of Its Function Domain

作　　者：王坤芃 彭志锋 刘盼盼 王继洋[1] 王艳[1] 王川庆[1] 杨霞[1] WANG Kunpeng1 , PENG Zhifeng2 , LIU Panpan1 , WANG Jiyang 1 , WANG Yan1 WANG Chuanqing1 ,YANG Xia1.(1. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China; 2. College of VeterinalT Medicine, Henan University of Animal Husband and Economy, Zhengzhou 450002, Chin)

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]河南牧业经济学院动物医学院,河南郑州450002

出　　处：《河南农业科学》2018年第3期138-143,共6页Journal of Henan Agricultural Sciences

基　　金：河南省自然科学基金资助项目(162300410153);河南省高校科技创新团队与支持计划资助项目(14IRTSHN015);国家自然科学基金项目(U1404328)

摘　　要：为了解鸭源鸡杆菌(G.anatis)菌毛蛋白Flf A的分布情况及功能,对18株G.anatis中国分离株的flfA基因进行扩增和分子克隆,应用相关软件对其核苷酸序列及氨基酸序列进行生物信息学分析。结果显示,18株G.anatis均具有flfA基因,核苷酸同源性为70.2%~99.8%,氨基酸同源性为63.1%~98.8%,有6~8个B细胞抗原表位,且这些优势的抗原表位多位于保守序列区,表明G.anatis不同菌株间可能存在交叉免疫反应。The aim of this study was to understand the distribution and function of fimbriae Flf A protein of G. anatis. flfA genes were amplified from 18 G. anatis isolates,followed by molecular cloning,bioinformatics analyses of flfA nucleotide and amino acid sequences with bioinformatics tools. The results showed that the nucleotide sequences homologies of 18 G. anatis isolates were 70. 2% —99. 8% and the deduced amino acid homologies were 63. 1% —98. 8%. FlfA protein has 6—8 B cell epitopes and most of them were located in the conserved sequence region. These results indicate that different strains may show immune cross protection.

关 键 词：鸭源鸡杆菌 flfA基因 克隆 序列分析 

分 类 号：S856[农业科学—临床兽医学]