大豆光周期效应GmGBP1基因启动子多态性分析  

Analysis of polymorhpism in GmGBP1 promoter involved in soybean photoperiod response

作　　者：赵琳[1] 姚愈恒 王阔 郑艳红 李英华 李敏敏[1] 张可欣[1] 杨雪[1] 丁福全[1] 李文滨[1] ZHAO Lin;YAO Yu -heng;WANG Kuo;ZHENG Yan -hong;LI Ying- hua;LI Min - min;ZHANG Ke - xin;YANG Xue;DING Fu - quan;LI Wen - bin(Key Laboratory of Soybean Biology in Chinese Ministry of Education/Key Laboratory of Soybean Biology and Breeding （Genetics） of Chinese Agriculture Ministry, Northeast Agricultural University, Harbin, 150030)

机构地区：[1]东北农业大学大豆生物学教育部重点实验室/农业部东北大豆生物学与遗传育种重点实验室,黑龙江哈尔滨150030

出　　处：《中国油料作物学报》2018年第2期174-180,共7页Chinese Journal of Oil Crop Sciences

基　　金：黑龙江省普通本科高等学校青年创新人才培养计划(UNPYSCT-2015003)

摘　　要：GmGBP1基因是植物开花途径中的重要基因,其启动子也受到短日照条件的强烈诱导。但是目前大豆GmGBP1基因启动子序列的多态性变异以及其与开花期的关系还不清楚。本试验对开花习性不同的36份大豆种质资源的GmGBP1基因启动子序列进行了克隆测序,发掘了GmGBP1基因启动子序列的自然等位变异,探讨了GmGBP1基因启动子序列的自然变异与开花期的关系。研究结果表明,多态性位点SNP-796与开花期密切相关,其中SNP-796G为缩短大豆生育时期的优异等位变异。主要的3个单倍型与开花期也表现出极显著(P<0.01)的相关性,单倍型2和单倍型3为缩短大豆花期的优异单倍型。Flowering period is one of the most important ecological traits of adaptation in soybean and controlled by multiple genes. Molecular biology studies have shown that the diversity of soybean flowering period controlled by photoperiod is attributed to the variation of photoperiod-related genes and their combination of variation. GmGBP1 is an important gene in the flowering process of plants and its promoter is also strongly induced by short day conditions. However,at present,the relationship between polymorphism of the promoter sequence of soybean GmGBP1 and its flowering time is still unclear. In this study,the promoter sequences of GmGBP1 from 36 soybean germplasms with different flowering habits were cloned and sequenced,and the natural allelic variation of GmGBP1 promoter was explored for the natural variation of GmGBP1 promoter sequence relationship with flowering period. The results showed that SNP-796 was closely related to flowering time,of which SNP-796 G was an excellent allele for shortening soybean growth stage. The 3 major haplotypes also showed significant（ P 0. 01） correlation with flowering stage. Haplotypes 2 and 3 were shortened haplotypes in soybean flowering stage.

关 键 词：GmGBP1启动子 多态性 始花期 关联分析 

分 类 号：S565.1[农业科学—作物学]