Protective action of aspirin and its against endothelial Nlrp3 inflammasome activation in response to LPS stimuli  

作　　者：Xing ZHOU Yi-lin ZHONG Yan-jiao WU Yi HUANG Yang CHEN 

机构地区：[1]School of Pharmaceutical,Guangzhou University of Chinese Medicine [2]Department of Stomatology,The First Affiliated Hospital,Jinan University

出　　处：《中国药理学与毒理学杂志》2017年第10期984-984,共1页Chinese Journal of Pharmacology and Toxicology

基　　金：supported by National Natural Science Foundation of China(81603587,81603668);Science and Technology Development Plan of Guangdong Province(2017A020211016);Youth Medical Talent Fund of Guangzhou University of Chinese Medicine(QNYC20170105)

摘　　要：OBJECTIVE Recent studies have demonstrated that the Nlrp3 inflammasome serve as a central role in the pathogenesis of cardiovascular diseases and endothelial dysfunction occurs in association with several cardiovascular risk factors.Given the demonstrated anti-inflammatory effects of aspirin,the present study was designed to test whether aspirin diminish NLRP3 inflammasome activation and prevent endothelium injury and associated coronary artery damage during LPS.METHODS Mouse carotid arterial endothelial cells(CAECs)were cultured and treated with 0.1-3 mmol·L^(-1) of aspirin in response to LPS(2μg·mL^(-1))stimuli.After 24 h,the Nlrp3 inflammasome complexes consist of varied proteins were analyzed by WB.NO and T-AOC in the supernatant was detected by ELISA.Intracellular reactive oxygen species(ROS)generation for 24 h was observed by DCF fluorescence.The mice were treated with aspirin(12.5 mg·kg^(-1) per day,62.5 mg·kg^(-1) per day,125 mg·kg^(-1) per day)and dexamethasone(0.0182 mg·kg^(-1)per day)for 7 d.The level of IL^(-1)β,IL^(-1)8 protein was detected by ELISA.RESULTS Immunofluorescence results showed the colocalization of Nlrp3 with ASC or caspase 1decrease in a concentration-dependent manner.Meanwhile,the expression of Nlrp3 and caspase 1protein was decreased with the concentration of aspirin,but no changes the expression of ASC protein.Nlrp3 protein levels in CAECs were 0.33-0.8-fold and cle-caspase 1 protein levels in CAECs were 0.48-1-fold compared to those in LPS stimulation when treated with 0.1-3 mmol·L^(-1) aspirin for 24 h(P<0.01).Aspirin significantly antagonized the effect of LPS on NO(1.22-1.91-fold that of LPS stimulation,P<0.01)and T-AOC expression(1.02-1.90-fold that of LPS stimulation,P<0.01).As the different concentration of aspirin treated,the generation of ROS was 0.51-1.10-fold that of LPS stimulation(P<0.01).In vivo data shown the level of IL^(-1)β,IL^(-1)8 protein from serum are in concordance with the level of Nlrp3 inflammasome activation.CONCLUSION We conclude that aspirin haOBJECTIVE Recent studies have demonstrated that the Nlrp3 inflammasome serve as a central role in the pathogenesis of cardiovascular diseases and endothelial dysfunction occurs in association with several cardiovascular risk factors.Given the demonstrated anti-inflammatory effects of aspirin,the present study was designed to test whether aspirin diminish NLRP3 inflammasome activation and prevent endothelium injury and associated coronary artery damage during LPS.METHODS Mouse carotid arterial endothelial cells（CAECs）were cultured and treated with 0.1-3 mmol·L^（-1） of aspirin in response to LPS（2μg·mL^（-1））stimuli.After 24 h,the Nlrp3 inflammasome complexes consist of varied proteins were analyzed by WB.NO and T-AOC in the supernatant was detected by ELISA.Intracellular reactive oxygen species（ROS）generation for 24 h was observed by DCF fluorescence.The mice were treated with aspirin（12.5 mg·kg^（-1） per day,62.5 mg·kg^（-1） per day,125 mg·kg^（-1） per day）and dexamethasone（0.0182 mg·kg^（-1）per day）for 7 d.The level of IL^（-1）β,IL^（-1）8 protein was detected by ELISA.RESULTS Immunofluorescence results showed the colocalization of Nlrp3 with ASC or caspase 1decrease in a concentration-dependent manner.Meanwhile,the expression of Nlrp3 and caspase 1protein was decreased with the concentration of aspirin,but no changes the expression of ASC protein.Nlrp3 protein levels in CAECs were 0.33-0.8-fold and cle-caspase 1 protein levels in CAECs were 0.48-1-fold compared to those in LPS stimulation when treated with 0.1-3 mmol·L^（-1） aspirin for 24 h（P〈0.01）.Aspirin significantly antagonized the effect of LPS on NO（1.22-1.91-fold that of LPS stimulation,P〈0.01）and T-AOC expression（1.02-1.90-fold that of LPS stimulation,P〈0.01）.As the different concentration of aspirin treated,the generation of ROS was 0.51-1.10-fold that of LPS stimulation（P〈0.01）.In vivo data shown the level of IL^（-1）β,IL^（-1）8 protein from serum are in concordanc

关 键 词：Asporin Nlrp3 inflammasome CAECs ROS 

分 类 号：R96[医药卫生—药理学]