甘草甜素镁的合成及其辐射防护作用研究  

Study on the synthesis and anti-radiation activity of glycyrrhizin magnesium

作　　者：牟感恩 贾艺悦 龙伟[1] 李园园[1] 周则卫[1] Mu Ganen;Jia Yiyue;Long Wei;Li Yuanyuan;Zhou Zewei(Tianjn Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, 300192 Tianjin, China)

机构地区：[1]中国医学科学院北京协和医学院放射医学研究所天津市放射医学与核医学重点实验室,天津300192

出　　处：《国际放射医学核医学杂志》2018年第2期148-153,共6页International Journal of Radiation Medicine and Nuclear Medicine

基　　金：天津市自然科学基金重点项目（15JCZDJC33400）

摘　　要：目的 研究甘草甜素衍生物-甘草甜素镁（Gly-Mg）的合成及其辐射防护作用。方法 采用甘草甜素（Gly）与镁进行取代反应合成Gly-Mg化合物，并应用磁共振氢谱及质谱鉴定其结构。以137Cs γ射线照射的ICR小鼠为实验对象，将小鼠分为5组：单纯照射对照组（生理盐水）、Gly （50 mg/kg）给药照射组、Gly-Mg低、中、高剂量给药照射组（25 mg/kg、50 mg/kg、75 mg/kg）。7.8 Gy致死剂量照射小鼠，观察各组照射小鼠（12只/组）30 d存活率。6.0 Gy亚致死剂量照射小鼠进行体内抗辐射实验，计算各组小鼠（8只/组）脏器指数，并测定WBC计数、骨髓有核细胞数、骨髓DNA的含量和脾结节数。采用Student-Newman-Keuls检验进行多组间显著性差异分析，两组间比较采用t检验。结果 磁共振氢谱及质谱鉴定表明，成功合成化合物Gly-Mg。小鼠30 d存活率实验结果表明，Gly 50 mg/kg给药照射组、Gly-Mg低、中、高剂量给药照射组小鼠的存活率由单纯照射对照组的8.3%分别升高至25.0%、25.0%、33.3%、41.7%，Gly-Mg高剂量给药照射组小鼠平均存活天数明显延长，差异有统计学意义（t=3.418，P〈0.05）。小鼠体内抗辐射活性实验结果表明，与单纯照射组比较，Gly-Mg低、中、高给药照射组受照小鼠的胸腺指数（t=3.259、7.580、3.415，均P〈0.01）、脾指数、肝脏指数（t=4.615、1.797，均P〈0.05；t=3.341，P〈0.01）、性腺指数（t=1.826，P〈0.05；t=2.631、2.893，均P〈0.01）均有增高，其中胸腺指数和肝脏指数、性腺指数差异具有统计学意义；Gly-Mg中剂量给药照射组可显著提高WBC计数（t=2.888，P〈0.01）、骨髓有核细胞数（BMNC）（t=4.570，P〈0.05）、骨髓DNA含量（t=6.139，P〈0.01）和脾结节数（t=1.872，P〈0.05），差异均有统计学意义。结论 Gly-Mg合成步骤简单，易获得。Gly-Mg具有显著的辐射防护作用，有望开发成为辐射防护�Objective To explore the synthesis and anti-radiation activity of glycyrrhizin magnesium through classic animal experiments. Methods The Gly-Mg compound was synthesized by the substitution reaction of glycyrrhizin（Gly） and magnesium, and the structure was identified by magnetic resonance spectroscopy and mass spectrometry. ICR mice irradiated with 137Cs γ-rays were used as experimental subjects. The mice were divided into 5 groups：single-irradiation control group （physiological saline）, Gly （50 mg/kg） group, Gly-Mg low, medium and high dose administration groups （25 mg/kg, 50 mg/kg, 75 mg/kg）.The 30-day survival rate of mice irradiated with 7.8 Gy （lethal dose） and the 7-day radiation resistance of mice irradiated with 6.0Gy （sub-between groups. The t-test was used to compare the two groups. Results The Gly-Mg was successfully synthesized. After the irradiation of 7.8 Gy γ-ray emitted from 137Cs radiation resource, the survival rates of mice irradiated with 7.8 Gy and given Gly-Mg at 25, 50, and 75 mg/kg increased to 25.0%, 33.3%, and 41.7%, respectively. The average survival time was prolonged significantly compared with the control group（t=3.418, P〈0.05）. The thymus index（t=3.259, 7.580, 3.415, all P〈0.05）, spleen index, liver index（t=4.615, 1.797, both P〈0.05; t=3.341, P〈0.01）, gonadal index（t=1.826, P〈0.05; t=2.631, 2.893, both P〈0.01） were improved of all Gly-Mg irradiated groups. Thymus, liver, and gonad indexes were statistically significant in different groups. The medium dose group of Gly-Mg significantly increased the number of WBC（t=2.888, P〈0.01）, bone marrow n ucleated（t=4.570, P〈0.05）, percentages of DNA（t=6.139, P〈0.01）, and colony forming unit of spleen（t=1.872, P〈0.05） the difference was statistically significant. Conclusions The steps involved in Gly-Mg synthesis are simple, and raw materials are easily available. Gly-Mg showed a protective effect against damage induced by irradiation. It is expected to be developed in

关 键 词：甘草甜素 甘草甜素镁 辐射防护 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]