中国荷斯坦牛乳铁蛋白启动子区-131C>T和-28A>C位点SNP与泌乳性状、乳房炎和生产寿命的关联分析  

Association Analysis on the SNP of LF-131C>T and LF-28A>C with Milk Performance, Clinical Mastitis and Lifetime for Chinese Holstein(Bos taurus)

作　　者：王梦琦[1] 倪炜[1] 唐程 郭佳禾 张慧敏[1] 李明勋 杨章平[1] 毛永江[1] WANG Meng-Qi;NI Wei;TANG Cheng;GUO Jia-He;ZHANG Hui-Min;LIMing- Xun;YANG Zhang-Ping;MAO Yong-Jiang(College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Chin)

机构地区：[1]扬州大学动物科学与技术学院,扬州225009

出　　处：《农业生物技术学报》2018年第5期811-818,共8页Journal of Agricultural Biotechnology

基　　金：江苏省农业自主创新基金(No.CX(17)1005);国家自然科学基金(No.31372286);江苏省企业研究生工作站

摘　　要：乳房炎是危害奶牛业最严重的疾病,并对奶牛(Bos taurus)泌乳性能和生产寿命产生显著影响。为探索乳铁蛋白基因(lactoferrin gene,LF)5'非翻译区(untranslating region,UTR)多态性对荷斯坦牛生产性状的影响,本研究根据LF基因5'-UTR区序列,利用PCR直接测序法对低体细胞评分(somatic cell score,SCS)和高SCS样本各20个样本进行SNP筛查,在此基础上利用飞行质谱法对866头中国荷斯坦牛LF基因5'-UTR区-131C>T和-28A>C位点多态性进行检测,同时收集所检测牛只奶牛群体改良计划(dairy herd improvement,DHI)泌乳相关性状记录、临床乳房炎和生产寿命等信息,利用多因素方差分析法、生存分析的Cox回归等方法分析以上SNP位点对泌乳性状、临床乳房炎发生次数和生产寿命的影响。结果表明LF-131C>T位点对SCS和乳糖有极显著影响(P<0.01),LF-28A>C位点对日产奶量、乳脂率、蛋白率、乳糖和总固体的影响均达到极显著水平(P<0.01)。LF-28A>C位点对奶牛生产寿命有显著影响(P<0.05),CC基因型个体生产寿命显著低于AA基因型。LF-131C>T对生产寿命有影响,但差异不显著。本研究表明LF-131C>T和LF-28A>C对提高奶牛泌乳性能和延长生产寿命具有重要意义,该研究结果可为奶牛的抗病育种和提高经济效益提供科学依据。Clinical mastitis is a kind of the most serious disease with high incidence in dairy industry, and has been plagued in the development of dairy industry. It has significant effects on milk performance and lifetime which will cause huge economic losses in milk production process. It＇s very important to study the related genes about mastitis for the improvement of milking traits and elongation of lifetime of cows with molecular techniques, and scientific basis would be provided for the breeding of anti-disease and improvement of economic benefits of dairy farm. In order to verify the effects of lactoferrin gene polymorphism on the production traits of Chinese Holstein （Bos taurus）, SNPs of LF gene were selected by PCR and direct sequencing for 20 cows with low SCS and 20 cows with high SCS according to the sequence of 5＇ untranslating region （UTR） of LF gene. Finally, LF-131C〉T and LF-28A〉C for 866 Chinese Holstein cows were detected by flight mass spectrometry. The dairy herd improvement production recordings information including the number of test cow, ID number of test cow＇s father, date of test, related genealogical information, test-day milk yield, fat content, protein content, somatic cell score, lactose, total solid, milk urea nitrogen, clinical mastitis and productive life of tested cows were collected from the management system of dairy farm. The effects of LF-131C〉T and LF-28A〉C on milk performances and lifetime were analyzed by multi factor variance analysis and Cox regression. The results showed that the dominant genotype of LF-131C〉T and LF-28A〉C loci were TT and AA with the frequency of 0.813 and 0.580, respectively. The dominant genes were T and A, respectively, and their frequencies were 0.903 and 0.762, respectively. Two loci were in the state of Hardy-Weinberg Equilibrium. LF-131C〉T had extremely significant effects on SCS, lactose content and 305 days milk yield （P＜0.01）, the effects on test-day milk yield and protein content were close to significant level （0

关 键 词：中国荷斯坦牛  乳铁蛋白  泌乳性状  乳房炎  生产寿命 

分 类 号：S823[农业科学—畜牧学]