广州地区中老年男性RANK/RANKL/OPG和WNT信号通路基因多态性与髋部骨强度的关联分析  

Association of Genes in RANK/RANKL/OPG and WNT Signaling Pathways with Hip Bone Strength among Middle-Aged and Elderly Males in Guangzhou

作　　者：王希丹 肖苏妹 陈裕明[1] WANG Xi-dan;XIAO Su-mei;CHEN Yu-ming(School of Public Health, Sun Yat-sen University, Guangzhou 510080, Guangdong, China)

机构地区：[1]中山大学公共卫生学院,中国广东广州510080

出　　处：《生命科学研究》2018年第2期143-148,共6页Life Science Research

基　　金：国家自然科学基金资助项目(81273049;81400857);广东省自然科学基金资助项目(2014A030313132);广州市科技计划项目(201610010093);中山大学5010临床研究项目(2007032)

摘　　要：为了探讨RANK/RANKL/OPG和WNT信号通路中6个基因10个单核苷酸多态性位点与中老年男性髋部骨强度的关联,征集788名广州地区40岁以上男性为研究对象,采用Mass Array检测基因型,双能X线吸收仪测定股骨颈骨密度(bone mineral density,BMD)、横截面积(cross-sectional area,CSA)、平均皮质厚度(average cortical thickness,ACT)、剖面模数(section modulus,SM)和抗屈曲率(buckling ratio,BR),以多重线性回归分析各位点与骨表型的关联。结果发现:OPG基因rs3134069的C等位基因与BMD(β=-0.017,P=0.024)和ACT(β=-0.004,P=0.040)负相关;LRP5基因rs3736228的T等位基因与低BMD、CSA、ACT(β=-0.050^-0.003,P<0.050)和高BR(β=0.242,P=0.043)相关,rs491347的G等位基因与低BMD相关(β=-0.012,P=0.046)。研究结果支持了OPG和LRP5基因与中国中老年男性人群髋部骨强度相关。The association of genes in the RANK/RANKL/OPG and WNT signaling pathways with hip bone strength was investigated among the middle-aged and elderly males. A total of 788 males aged over 40 years were included in the study. They were recruited from Guangzhou, China. Ten single nucleotide polymorphisms in 6 genes were genotyped using MassArray. Bone mineral density （BMD）, cross-sectional area （CSA）, average cortical thickness （ACT）, section modulus （SM） and buckling ratio （BR） at the femoral neck were mea- sured by dual-energy X-ray absorptiometry. The relationships between the genes and bone phenotypes were detected using multiple linear regression analysis. The results showed that the minor allele C of rs3134069 in OPG gene was negatively associated with BMD and ACT （β =-0.017, P=-0.024 for BMD; β=-0.004, P=0.040 for ACT）. In the LRP5 gene, the minor allele T of rs3736228 was related with the lower BMD, CSA, ACT （β=0.050 --0.003, P〈0.050） and higher BR （β=0.242, P=0.043）, and the minor allele G of rs491347 was associ- ated with the reduction of BMD （β=-0.012, P=0.046）. This study supported that the OPG and LRP＇5 genes were associated with hip bone strength of the middle-aged and elderly males in Guangzhou.

关 键 词：RANK/RANKL/OPG信号通路 WNT信号通路 基因多态性 髋部骨密度 髋部骨几何结构 

分 类 号：348[医药卫生—内分泌] R589.5[医药卫生—内科学]