基于快速液相色谱技术对祛浊通痹复方质量控制的研究  

Research on Quality Control of Quzhuo Tongbi Fufang by RPLC

作　　者：谢志军[1] 严瑶琦 苗丽丽 邵丰 孙静 李海昌[1] 温成平[1] XIE Zhijun;YAN Yaoqi;MIAO Lili;et al(School of Basie Medical Sciences ,Zhejiang Chinese Medicine University ,Zhejiang,Hangzhou 310053, China.)

机构地区：[1]浙江中医药大学基础医学院,浙江杭州310053 [2]浙江中医药大学附属第二医院,浙江杭州310005

出　　处：《中国中医急症》2018年第5期773-776,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：浙江省自然科学基金项目(LQ16H270004);"十一五"国家科技支撑计划项目(2007BAI20B06)

摘　　要：目的测定祛浊通痹复方中落新妇苷、薯蓣皂苷元、姜黄素、槲皮素4种成分,建立祛浊通痹复方质量控制的方法。方法采用快速液相色谱法(RPLC)对复方中的落新妇苷、薯蓣皂苷元、姜黄素、槲皮素进行含量测定。结果落新妇苷、薯蓣皂苷元、姜黄素、槲皮素的线性范围分别为0.105~0.525μg(R2=0.9993)、0.637~3.185μg(R2=0.9998)、0.021~0.105μg(R2=0.9999)、0.0625~0.313μg(R2=0.9977),平均加样回收率分别为101.29%、97.35%、101.97%、99.18%。结论该方法准确,重现性和稳定性好,可用于祛浊通痹方的质量控制。Objective： To detect the levels of Astilbin,Diosgenin,Curcumin,Quercetin in Quzhuo Tongbi Fu- fang,and to establish its method of quality control. Methods： The content of Astilbin, Diosgenin, Curcumin and Quercetin in the formula were determined by the Rapid Performance Liquid Chromatographic （RPLC） technique. Results： The linear range of Astilbin, Diosgenin, Curcumin and Quercetin separately was 0.105-0.525 p.g （R2= 0.9993） ,0.637-3.185 μg（R2=0.9998）,0.021-0.105 μg（R2=0.9999） and 0.0625-0.313 μg（R2=0.9977）,respec- tively. The average plus sample reeovery rate was 101.29%, 97.35%, 101.97% and 99.18%, respectively. Conclu- sion： The method is aecurate,reprodueible and stable,and it can be used for quality control of Quzhuo Tongbi Fufang.

关 键 词：祛浊通痹复方 RPLC 落新妇苷 薯蓣皂苷元 姜黄素 槲皮素 

分 类 号：R284.1[医药卫生—中药学]