GC法测定用于生脉注射液中五味子药材挥发性成分指纹图谱的建立  

Determination of Fingerprint of Volatile Components in Schisandra Chinensis in Shengmai Injection by GC

作　　者：吴建国 董礼 李梅 徐小平[1] WU Jianguo;DONG Li;LI Mei;et al(West China School of Pharmacy,Siehuan University,Sichuan, Chengdu 610041, China)

机构地区：[1]四川大学华西药学院,四川成都610041 [2]华润三九(雅安)药业有限公司,四川雅安625000

出　　处：《中国中医急症》2018年第5期817-819,823,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：四川省科技支撑计划项目(2014SZ0139)

摘　　要：目的建立用于生脉注射液的五味子药材挥发性成分指纹图谱方法。方法采用水蒸气蒸馏法结合固相萃取法提取五味子药材挥发性成分;利用GC法建立指纹图谱检测方法;通过GC-MS联用技术确认指纹图谱中参照物峰;并在生脉注射液中验证五味子药材挥发性成分指纹图谱的控制意义。结果五味子挥发性成分指纹图谱中有9个特征峰,其中5、6号峰分别为4-萜品醇和α-松油醇;生脉注射液中存在五味子药材挥发性成分指纹图谱信息。结论 GC法用于生脉注射液中五味子挥发性成分指纹图谱建立方法稳定可靠,指纹信息在生脉注射中显著,能有效控制生脉注射液中五味子挥发性成分质量。Objective： To establish an method for determining fingerprint of volatile components in Schisandra chinensis in Shengmai Injection. Methods： The volatile components was extracted by the combined methods of steam distillation and solid phase extraction. The fingerprint was determined by GC. And the reference peaks were confirmed by GC-MS. And there was a verification of fingerprint of volatile components in Schisandra chinensis in Shengmai Injectionin. Results ： There were nine character peaks in fingerprint of volatile components in Schisan- dra chinensis. The fifth and sixth peak were Terpinen-4-ol and c^-Terpineol. The fingerprint of volatile compo- nents in Schisandra chinensis existed in Shengmai Injection. Conclusion： The method of determining fingerprint of volatile components in Schisandra chinensis in Shengmai Injection is stable and reliable. Fingerprint informa- tion is significant in Shengmai Injection and can effectively control the quality of volatile components in Schisan- dra chinensis in Shengmai Injection.

关 键 词：指纹图谱 生脉注射液 水蒸气蒸馏 固相萃取 

分 类 号：R284.1[医药卫生—中药学]