丹参注射液促进人脱落乳牙牙髓干细胞成骨/成牙分化的实验研究  

Salvia Miltiorrhiza enhanced the osteo/dentinogenic differention of stem cells from human exfoliated deciduous teeth

作　　者：高润涛[1] 范志朋[2] GAO Run-tao;FAN Zhi-peng(Department of Stomatology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China)

机构地区：[1]首都医科大学附属北京友谊医院口腔科,北京100050 [2]首都医科大学口腔医学院口腔医学研究所

出　　处：《北京口腔医学》2018年第2期65-68,共4页Beijing Journal of Stomatology

基　　金：国家自然科学基金(81271164);北京中医药科技发展资金(JJ2015-10)

摘　　要：目的探讨中药丹参对人脱落乳牙牙髓干细胞(SHEDs)成骨/成牙分化功能的影响。方法利用50mg/ml的丹参注射液作用于SHEDs细胞,利用成骨/成牙分化诱导培养基诱导SHEDs定向分化。通过检测ALP活性、茜素红染色、钙离子定量分析、成骨/成牙分化相关基因的表达研究SHEDs体外成骨/成牙分化能力。Western Blot检测AKT信号分子的表达。结果 50mg/ml的丹参注射液促进SHEDs细胞ALP活性,体外矿化能力及成骨/成牙分化相关基因骨涎蛋白(bone sialoprotein,BSP)、牙本质涎磷蛋白(Dentin sialophosphoprotein,DSPP)的表达。50mg/ml的丹参注射液促进磷酸化AKT的表达。结论 50mg/ml的丹参注射液具有促进SHEDs细胞体外成骨/成牙分化的潜能,其作用可能与激活AKT信号通路相关。Objective To investigate the effect of Salvia Miltiorrhiza on the osteo/dentinogenic differentiation of stem cells from human exfoliated deciduous teeth（ SHEDs）. Methods SHEDs were induced to osteo/dentinogenic differentiation by using osteogenic-inducing medium and 50 mg/ml Salvia Miltiorrhiza. ALP activity assay,alizarin-red staining,quantitative analysis of calcium and osteo/dentinogenesis related genes expression were detected for osteo/dentinogenic differentiation capacity. Western blot analysis was used to examine the AKT signaling pathway. Results 50 mg/ml Salvia Miltiorrhiza enhanced ALP activity,mineralization,the expression of bone sialoprotein（ BSP） and dentin sialophosphoprotein（ DSPP） in SHEDs.50 mg/ml Salvia Miltiorrhiza increased the expression levels of phosphorylated AKT（ p-AKT） in SHEDs. Conclusion Salvia Miltiorrhiza could enhance the osteo/dentinogenic differentiation of SHED,which may be related to AKT signaling pathway.

关 键 词：脱落乳牙牙髓干细胞 丹参 成骨/成牙分化 

分 类 号：R349.5[医药卫生—基础医学]