内皮脂酶与重度子痫前期的相关性及其对滋养层细胞功能影响的研究  

Relation between preeclampsia and EL and effects on HTR8 by down-regulating EL

作　　者：陈圆圆[1] 程蔚蔚[1] Chen Yuanyuan;Cheng Weiwei(The International Peace Maternity and Child Health Hospital, Shanghai 20003)

机构地区：[1]上海交通大学医学院附属国际和平妇幼保健院,上海200030

出　　处：《现代妇产科进展》2018年第4期241-244,共4页Progress in Obstetrics and Gynecology

基　　金：国家自然科学基金资助项目(No:81370727);上海市卫生和计划生育委员会科研课题计划(No:201640361)

摘　　要：目的:探讨内皮脂酶(EL)基因在重度子痫前期(PE)患者胎盘组织中的表达,以及EL对人胎盘滋养层细胞生物学功能的影响及其调控机制。方法:收集12例重度PE患者和12例正常孕妇的胎盘组织。Real time-PCR、Western blot法检测EL mRNA和蛋白水平表达。脂质体转染人正常绒毛膜滋养层细胞系HTR8/SVneo干扰EL表达,Real timePCR、Western blot法检测EL转录及表达水平。用Annexin V-FITC、Transwell、Invasion、Tube formation等检测EL表达降低对HTR8/SVneo细胞凋亡、侵袭、迁移及血管化等能力的影响。Real time-PCR探究HTR8/SVneo功能改变的分子机制。结果:PE胎盘组织中EL表达水平下降;EL表达下降影响滋养细胞凋亡、侵袭、迁移等生物活性,MMP-9表达降低。结论:EL可能通过改变MMP-9表达影响人滋养层细胞的生物学功能,并通过S1PR-SPHK-e NOS通路参与胎盘血管的浅着床过程,从而促进PE的发生发展。Objective： To research the differential expression of EL in placenta both in normal and PE women,and to investigate the affective mechanism of EL on human trophoblast cell.Methods： 12 pairs of placenta tissues were collected and Real-time PCR、Western blot were used to research whether EL changed in PE. The HTR8 down-expressing EL was got through transfecting HTR8 with siRNA-EL.Flow cytometry test,Cell scratch test were used to investigate the influence of EL on many functions of HTR8 such as proliferation,migration was evaluated.To investigate the molecular mechanism of EL decrease by Real-time PCR.Results： EL was decreased in placenta of PE.The apoptosis rate,migration also declined.The expression of MMP-9 decreased.Conclusion： The bionomics of HTR8 decrease as EL expression decline,these changes may participate in the pathophysiology of PE. EL expression may affect the expression of MMP-9,which may bring about the inhibitory of trophoblast cells implantation.

关 键 词：子痫前期 内皮脂酶 胎盘滋养层细胞 凋亡 迁移 

分 类 号：R714.25[医药卫生—妇产科学]