过量表达RdreB1BI对草莓果实品质及相关基因的影响  

Effect of RdreB1BI Gene Overexpression on Fruit Quality and Related Gene Expression in Strawberry

作　　者：阎依超 万春雁[2] 古咸彬 郭成宝 陈月红 高志红[1] YAN YiChao;WAN ChunYan;GU XianBin;GUO ChengBao;CHEN YueHong;GAO ZhiHong(Nanjing Agricultural University, Nanjing 210095;Zhenjiang Institute of Agricultural Science, Zhenjiang 212400, Jiangsu;Nanjing Institute of Agricultural Science, Nanjing 210000)

机构地区：[1]南京农业大学,南京210095 [2]江苏丘陵地区镇江农业科学研究所,江苏镇江212400 [3]南京市农业科学研究所,南京210000

出　　处：《中国农业科学》2018年第7期1353-1367,共15页Scientia Agricultura Sinica

基　　金：江苏省农业科技自主创新资金项目(CX(12)5065);江苏省六大人才高峰人才项目(NY068)

摘　　要：【目的】研究外源基因Rdre B1BI转入对‘红颊’草莓果实品质及相关基因表达的影响,揭示Rdre B1BI在草莓果实品质调控中的作用及分子机理。【方法】以5个转Rdre B1BI株系及非转基因‘红颊’草莓全红期果实为材料,测定果实纵横径、单果重、可溶性糖、可溶性蛋白、抗坏血酸等风味物质及花青苷、总黄酮、总酚等着色物质的含量。应用BLASTn、GENEFINDER和Plant CARE等生物信息学方法分析外源基因和7个次生代谢产物合成途径相关基因(Rdre B1BI、Fractin、Fv C4H、Fv CCR2、Fv GST、Fv F3H、Fv DFR和Fv MYB306)的结构,并预测基因启动子作用元件。采用q RT-PCR定量法检测相关基因表达量,应用7300 system软件和2-△△Ct法分析数据。对生理生化及分子数据进行方差及相关性分析,综合讨论Rdre B1BI的转入对‘红颊’草莓果实品质及相关基因表达的影响。【结果】转基因‘红颊’草莓株系与野生型果实单果重的范围为11.75—15.42 g,纵径和横径的范围分别为35.12—40.42 mm及28.73—32.6 mm,仅株系8果实纵径显著大于株系7,其余指标间差异不显著。其中转基因株系1和7的花青苷含量显著高于野生型;转基因株系1、7及8总黄酮含量显著高于野生型;各转基因株系的总酚含量均显著高于野生型。转基因株系1、7和8果实的可溶性糖含量显著高于野生型(21.70 mg·g-1FW),分别为野生型的2.87、3.39和3.35倍。可溶性固形物含量与可溶性糖含量呈正相关(r=0.811*),但样品果实的可溶性固形物含量间不存在显著性差异。转基因株系草莓成熟果实果肉中氨基酸含量为0.2580—0.3950 g/100 g FW,野生型果实的氨基酸含量为0.5151 g/100 g FW,显著高于各转基因株系草莓果实;转基因株系1和7的可滴定酸含量显著高于野生型;转基因株系1果实的抗坏血酸含量为168.35 mg/100 g FW,显著高于野生型(92.50 mg/100 g FW)。转基因株系9及10果实的可溶�【Objective】 The effect of exogenous Rdre B1 BI transformation to the fruit quality and related gene expression in ‘Benihoppe＇ strawberry were analyzed to reveal the function and molecular mechanism of quality regulation in transgenic strawberry fruit. 【Method】 The edible organs on five Rdre B1 BI transgenic strawberry lines and non-transgenic ‘Benihoppe＇ strawberries（fruits in full red stage） were used as material to test and determine the vertical diameter, transverse diameter, weight, contents of flavor substance（soluble sugar, soluble protein, ascorbic acid） and coloring materials（anthocyanin, flavonoids and total phenol）. The structure of the exogenous gene and seven secondary metabolites synthesis pathway-related genes（Rdre B1 BI, Fractin, Fv C4 H, Fv CCR2, Fv GST, Fv F3 H, Fv DFR and Fv MYB306） and the elements of gene promoter were analyzed and were predicted by bioinformatics methods such as BLASTn, GENEFINDER, and Plant CARE. The expression of related genes was detected by q RT-PCR. The data were analyzed by 7300 system software and 2-△△Ct method. The variance and correlation analysis of physiological, biochemical and molecular data were analyzed. The effects of Rdre B1 BI on the fruit quality and related gene expression of ‘Benihoppe＇ strawberry were discussed comprehensively. 【Result】 The fresh weight of transgenic and non-transgenic strawberries was ranging from 11.75 to 15.42 g. The vertical and transverse diameters were ranging from 35.12 to 40.42 mm and 28.73 to 32.6 mm, respectively. Only the volume diameter of transgenic line 8 was significantly higher than that of line 7, and there were no significant differences between the other samples. The contents of anthocyanin in line 1 and line 7 were significantly higher than those of the control. The contents of total phenol in transgenic line1 and line 7 were significantly higher than that in control. The contents of total phenol in each line were significantly higher than that in the control. The soluble su

关 键 词：草莓 RdreB1BI 过量表达 果实品质 启动子 

分 类 号：S668.4[农业科学—果树学]