榆耳菌丝原生质体制备及再生的研究  

Optimization of Preparation and Regeneration Conditions of Gloeostereum incarnatum Mycelium Protoplast

作　　者：刘宏宇 郭鹏程 姚方杰[1] LIU Hongyu;GUO Pengcheng;YAO Fangjie(Jilin Agricultural University, Changchun 130118, China)

机构地区：[1]吉林农业大学,长春130118

出　　处：《东北农业科学》2018年第2期60-64,共5页Journal of Northeast Agricultural Sciences

基　　金：吉林省高等学校经济菌物高端科技创新平台项目(吉高平合字[2014]B-1)

摘　　要：对珍稀食药用菌榆耳的原生质体制备与再生的条件进行优化,明确了原生质体制备的最佳条件:采用培养时间为7 d的菌丝,使用0.6 mol/L的甘露醇溶液作为稳渗剂配制的浓度为2%的溶壁酶溶液,在30℃的恒温摇床中70 r/min酶解3 h,原生质体产率最高。用RM4配方,28℃条件下的再生率最高,最高再生率可达1.83%。The article working on optimized the protoplast preparation and regeneration conditions of rare edible fungi Gloeostereum incarnatum. The conditions are defined, use mycelium cultured 7 d, and 0.6 mol/L mannitol solution was used as Osmotic stabilizer to make lywallzyme solution of 2%.At 30℃, 70 r/min Enzymatic hydrolysis of 3 hours, the largest protoplasts yield can be obtained. The regeneration rate of RM4 medium at 28℃ was the highest,regeneration rate could reach 1.83%.

关 键 词：榆耳 原生质体 制备与再生 

分 类 号：S567.3[农业科学—中草药栽培]