基于HPLC特征图谱研究不同生长阶段麦冬有效组分积累特征  

Study on accumulation rules of effective components in Ophiopogonis Radix of different growth phase based on HPLC specific chromatogram

作　　者：吴发明[1,2] 王盼[1] 李敏[1] 姚尹伊 包小红 周娟 WU Fa-ming;WANG Pan;LI Min;YAO Yin-yi;BAO Xiao-hong;ZHOU Juan(Chengdu University of TCM, The Ministry of Education Key Laboratory of Standardization of Chinese crude drug, State Key Laboratory Breeding Base of Systematic Research Development and Utilization of Chinese Medicines Resources Co-founded by Sichuan Province and MOST, Chengdu 611137, China;Zunyi Medical University, Zunyi 563003, China;Sichuan Institute for Food and Drug Control, Chengdu 611000, China)

机构地区：[1]成都中医药大学中药材标准化教育部重点实验室,中药资源系统研究与开发利用国家重点实验室,四川成都611137 [2]遵义医学院,贵州遵义563003 [3]四川省食品药品检验检测院,四川成都611000

出　　处：《中草药》2018年第7期1682-1687,共6页Chinese Traditional and Herbal Drugs

基　　金：川产道地药材麦冬、半夏的品种和质量评价研究(2014F038); 四川三台县涪城麦冬集成技术推广与应用科技惠民项目(2013GS510102); 中药质量安全检验检测和风险控制技术平台(2014ZX09304307-002); 麦冬整合式产业链综合开发研究(2015SZ0033)

摘　　要：目的研究麦冬块根发育过程中有效组分的积累规律,为麦冬生产和质量控制提供理论支持。方法采用高效液相色谱法进行分析。DAD检测器:柱温30℃,检测波长203 nm,进样量15μL,以乙腈(A)-水(B)为流动相梯度洗脱,0~20 min,25%~40%A;20~50 min,40%~50%A;50~60 min,50%~70%A;60~70 min,70%A;70~80 min,70%~100%A,80~90 min,100%A,体积流量1.0 mL/min。ELSD检测器:柱温35℃,漂移管温度100℃,气体体积流量3.0 L/min;进样量15μL,流动相为乙腈(A)-0.1%磷酸水(B)溶液,梯度洗脱:0~60 min,35%~65%A,体积流量1.0 mL/min。结果 2种不同检测条件色谱图中主要代表组分均能较好分离,203 nm条件下有14个有效峰,主要峰为黄酮类成分,ELSD条件下有11个有效峰,主要峰为皂苷类成分,黄酮类成分中以麦冬甲基黄烷酮A为代表,皂苷类成分中以麦冬皂苷D为代表,根据峰面积可以判断出麦冬药材中各组分在不同生长阶段其积累特征各不相同,但其整体变化特征呈增大至平衡后小幅下降趋势。结论 HPLC特征图谱能够比较全面地反映麦冬块根有效组分积累特征,可为指导麦冬生产和药材质量控制提供丰富的信息。Objective To explore the change features of Ophiopogonis Radix in the development process, and to find the accumulation rules of effective components in Ophiopogonis Radix. Methods The method of chlorfluazuron formulation was analyzed by HPLC. DAD detector, column temperature was set at 30 ℃ and detection wavelength was 203 nm; Flow rate was set at 0.8 mL/min and sample size was 15 μL and the mobile phase was acetonitrile（A）-water（B） with gradient elution mode（0-20 min, 25% A →40% A; 20-50 min, 40%-50% A; 50-60 min, 50%-70% A; 60-70 min, 70% A; 70-80 min, 70%-100% A, 80-90 min, 100% A）, at the volume flow rate of 1.0 mL/min. ELSD detector, column temperature was set at 35 ℃, drift tube temperature at 100 ℃ and the gas flow rate was set at 3.0 L/min. The sample size was 15 μL and the mobile phase was acetonitrile（A）-0.1% phosphoric acid water（B） with gradient elution mode： 0-60 min, 35%-65% A, at the volume flow rate of 1.0 mL/min. Results The main components in the chromatogram were both separated well under the two conditions. At 203 nm, there were 14 effective peaks, the main components were flavones whose representative was methylophiopogonanone A. There were 11 effective peaks in the condition of ELSD, and the main peak represent saponins whose representative was Ophiopogonin D. It could be judged that the accumulation law of the each component in Ophiopogonis Radix was inconsistent in the different growth phase according to the peaks area. The overall change tendency of the components is increase to peak value and keep balance and to a slight decrease after the balance. Conclusion The accumulation rules of effective components in Ophiopogonis Radix could be reflected comprehensively by HPLC specific chromatogram.It could provide abundant information for the produce of Ophiopogonis Radix and quality control.

关 键 词：麦冬 HPLC 皂苷 黄酮 麦冬甲基黄烷酮A 麦冬皂苷D 

分 类 号：R286.6[医药卫生—中药学]