Strong contact coupling of neuronal growth cones with height-controlled vertical silicon nanocolumns  

作　　者：Seong-Min Kim Seyeong Lee Dongyoon Kim Dong-Hee Kang Kisuk Yang Seung-Woo Cho Jin Seok Lee Insung S. Choi Kyungtae Kang Myung-Han Yoon 

机构地区：[1]School of Materials Science and Engineering, Gwangju Institute of Science and Technology, Gwongju 61005, Republic of Korea [2]Department of Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea [3]Department of Chemistry, Sookmyung Women's University, Seoul 04310, Republic of Korea [4]Center for Cell-Encapsulation Research, Department of Chemistry, KAIST, Daejeon 34141, Republic of Korea [5]Department of Applied Chemistry, Kyung Hee University, Yongin, Gyeonggi 17104, Republic of Korea

出　　处：《Nano Research》2018年第5期2532-2543,共12页纳米研究（英文版）

摘　　要：In this study, we report that height-controlled vertically etched silicon nano- column arrays （vSNAs） induce strong growth cone-to-substrate coupling and accelerate in vitro neurite development while preserving the essential features of initial neurite formation. Large-scale preparation of vSNAs with flat head morphology enabled the generation of well-controlled topographical stimulation without cellular impalement. A systematic analysis on topography- induced variations on cellular morphology and cytoskeletal dynamics was conducted. In addition, neurite development on the grid-patterned vSNAs exhibited preferential adhesion to the nanostructured region and outgrowth directionality. The arrangement of cytoskeletal proteins and the expression of a focal adhesion complex indicated that a strong coupling existed between the underlying nanocolumns and growth cones. Furthermore, the height-controlled nanocolumn substrates differentially modulated neurite polarization and elongation. Our findings provide an important insight into neuron-nanotopography interactions and their role in cell adhesion and neurite development.In this study, we report that height-controlled vertically etched silicon nano- column arrays （vSNAs） induce strong growth cone-to-substrate coupling and accelerate in vitro neurite development while preserving the essential features of initial neurite formation. Large-scale preparation of vSNAs with flat head morphology enabled the generation of well-controlled topographical stimulation without cellular impalement. A systematic analysis on topography- induced variations on cellular morphology and cytoskeletal dynamics was conducted. In addition, neurite development on the grid-patterned vSNAs exhibited preferential adhesion to the nanostructured region and outgrowth directionality. The arrangement of cytoskeletal proteins and the expression of a focal adhesion complex indicated that a strong coupling existed between the underlying nanocolumns and growth cones. Furthermore, the height-controlled nanocolumn substrates differentially modulated neurite polarization and elongation. Our findings provide an important insight into neuron-nanotopography interactions and their role in cell adhesion and neurite development.

关 键 词：vertical silicon nanostructures metal-assisted chemical etching primary hippocampal neurons neurite outgrowth cytoskeletal dynamics 

分 类 号：Q245[生物学—细胞生物学] S661.2[农业科学—果树学]