云南省保山市蚊虫中检测到基因Ⅰ型流行性乙型脑炎病毒  

Identification of Japanese encephalitis virus genotype Ⅰ from mosquitoes in Baoshan,Yunnan

作　　者：张婧 李加全[3] 冯云[1] 韩茜[1] 潘虹[1] 杨和仙[3] 张海林[1] 宋志忠[4] Zhang Jing;Li Jiaquan;Feng Yun;Hart Xi;Pan Hong;Yang Hexian;Zhang Hailin;Song Zhizhong(Yunnan Institute of Endemic Diseases Control and Prevention, Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Dali 671000, Yunnan, China;Public Health College, Dali University, Dali 671000, Yunnan, China;Baoshan Prefecture Center for Disease Control and Prevention, Baoshan 678000, Yunnan, China;Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, Yunnan, China)

机构地区：[1]云南省地方病防治所,云南省自然疫源性疾病防控技术重点实验室,云南大理671000 [2]大理大学公共卫生学院,云南大理671000 [3]保山市疾病预防控制中心,云南保山678000 [4]云南省疾病预防控制中心,云南昆明650022

出　　处：《疾病监测》2018年第2期150-154,共5页Disease Surveillance

基　　金：国家自然科学基金(No.31560049);云南省应用基础研究计划(No.2016FB029)~~

摘　　要：目的调查云南省保山市蚊虫及其自然感染虫媒病毒状况,为蚊媒病毒病防控提供参考依据。方法 2015年9月采用诱蚊灯法在保山市隆阳区农村人房和畜圈采集蚊虫标本,反转录聚合酶链反应(RT-PCR)用于检测蚊虫标本中黄病毒属、甲病毒属、布尼亚病毒属和流行性乙型脑炎病毒(乙脑病毒)等相关病毒核酸,阳性者进行C/pr M基因序列测定、同源性和系统进化分析。结果共捕获蚊虫3种8 202只,三带喙库蚊(Culex tritaeniorhynchus)、中华按蚊(Anopheles sinensis)和骚扰阿蚊(Armigeres subalbatus)构成比依次为88.50%(7 259只)、11.43%(937只)和0.07%(6只)。蚊虫标本分30批进行RT-PCR检测,14批三带喙库蚊中检测到乙脑病毒核酸,并获得14株乙脑病毒C/pr M基因核苷酸序列。同源性和进化分析表明,14株乙脑病毒与基因Ⅰ型乙脑病毒同在一个进化分支,并与2007年保山市腾冲县和2010年德宏傣族景颇族自治州(芒市和瑞丽市)的基因Ⅰ型乙脑病毒具有较近的进化关系。结论三带喙库蚊为保山市的优势蚊种和乙脑病毒主要传播媒介,当地存在基因Ⅰ型乙脑病毒流行,此为近10年保山市首次检测到基因Ⅰ型乙脑病毒。Objective To investigate the distribution of mosquitoes and their natural infection with mosquito-borne viruses in Baoshan of Yunnan province, and provide scientific evidence for the prevention and control of mosquito-borne diseases. Methods Mosquito samples were collected by using mosquito traps in the night from houses and livestock stables in villages in Baoshan in September 2015. The mosquito samples were used to detect the viral RNA with Reverse transcription- polymerase chain reaction （RT-PCR） using Flavivirus, Alphavirus, Bnnyavirus and Japanese encephalitis virus （JEV） primers, and positive specimens were sequenced for C/prM gene for homology and phylogenetic analysis. Results A total of 8 202 mosquitoes in 3 species were collected in Longyang district of Baoshan. Culex tritaeriorhynchus, Anopheles sinensis and Armigeres subalbatus accounted for 88.50% （7 259）, 11.43% （937） and 0.07% （6） of the total, respectively. Thirty mosquito batches were detected by using RT-PCR, and JEV RNA were detected from 14 batches of Cx. tritaeriorhynchus. The sequences of C/prM genes of the 14 JEV strains were obtained. Homology and phylogenetic analysis showed that the 14 JEV strains were in the same clade of genotype I （G- I ） strains of JEV from GenBank, indicating they belonged to G- I , and all the JEV strains detected were closely related to the JEV G- ~ strains from Tengchong county of Baoshan in 2007 and adjacent Mangshi and Ruili counties of Dehong in 2010. Conclusion Cx. tritaeriorhynchus was dominant mosquito species and the major transmitting vector of JEV in Baoshan. JEV G- I was confirmed to circulate in the area. It was first time to detect JEV G- I in Baoshan since 2007.

关 键 词：蚊虫 虫媒病毒 流行性乙型脑炎病毒 基因I型 

分 类 号：R512.32[医药卫生—内科学]