环孢素A预处理对离体大鼠心肌细胞凋亡及心脏乳酸脱氢酶和Na^+,K^+-ATP酶活性的影响  

Effect of Cyclosporine A Pretreatment on Cardiomyocyte Apoptosis Rate,Cardiac Lactate Dehydrogenase and Activity of Na^+,K^+-ATP Enzyme in Isolated Rat Hearts

作　　者：陈璐 赵鑫 Chen Lu;Zhao Xin(Department of Anesthesiology, The Second Hospital Affiliated to Suzhou University, Jiangsu, Suzhou, China 215004;Department of Anesthesiology, Inner Mongolia Autonomous Region Tumor Hospital, Hohhot, Inner Mongolia, China 010020)

机构地区：[1]苏州大学附属第二医院麻醉科,江苏苏州215004 [2]内蒙古自治区肿瘤医院麻醉科,内蒙古呼和浩特010020

出　　处：《中国药业》2018年第10期12-15,共4页China Pharmaceuticals

摘　　要：目的探讨环孢素A预处理对离体大鼠心脏乳酸脱氢酶活性、心肌细胞的凋亡及Na^+,K^+-ATP酶活性的影响。方法选择健康雄性Wistar大鼠48只,按随机数字表法分为对照组(A组),缺血再灌注组(B组)和环孢素A组(C组),各16只。所有大鼠均制备Langendorff离体心脏灌注模型,A组大鼠利用Krebs液进行全心灌流90 min;B组大鼠先用Krebs液进行灌注,使心脏保持在正常工作状态30 min,然后切换成缺血状态30 min,再进行灌注30 min;C组大鼠先用Krebs液进行灌注10 min,然后换成浓度为0.1 mmol/L环孢素A的K-B液进行灌注,心脏在持续给药状态下保持正常工作20 min,然后切换成缺血状态30 min,再灌注30 min。结果 3组大鼠的乳酸脱氢酶活性、心肌梗死面积百分比、Na^+,K^+-ATP酶活性相比,差异均有统计学意义(F=42.533,366.713,46.279,P<0.05),q检验结果显示,任意两组之间的差异均有统计学意义(P<0.05),测定数值均为B组>C组>A组。结论环孢素A预处理可降低缺血-再灌注损伤后心肌细胞乳酸脱氢酶的活性,减少心肌细胞的凋亡,降低Na^+,K^+-ATP酶活性,对心肌缺血-再灌注损伤有一定防护作用。ObjectiveTo investigate the effect of cyclosporine A pretreatment on cardiomyocyte apoptosis rate（CMAR）,cardiac lactate dehydrogenase（LDH）and activity of Na^＋,K^＋-ATP enzyme in isolated rat hearts.Methods Totally 48 healthy male Wistar rats were selected and divided into the control group（group A）,ischemia reperfusion group（group B）and cyclosporin A group（group C）according to the random number table method,16 rats in each group.All rats were prepared Langendorff isolated cardiac perfusion models.The rats in group A were fully perfused with Krebs solution for 90 min.The rats in group B were perfused with Krebs fluid firstly to keep the heart in normal working condition for 30 min and then switched to ischemic state for 30 min,followed by perfusion for 30 min.The rats in group C were perfused with Krebs solution for 10 min and then perfused with K-B solution at a concentration of 0.1 mmol/L cyclosporine A.The heart remained in normal state for 20 min after continuous administration,and then switched to ischemic state for 30 min,followed by perfusion for 30 min.ResultsThe activities of LDH,percentages of myocardial infarct area,activity of Na^＋,K^＋-ATP enzyme in the three groups were statistically significant（F=42.533,366.713,46.279,P〈0.05）.q test results showed that the difference between any two groups was statistically significant（P〈0.05）,and the measured values were as follows：group B〉group C〉group A.Conclusion Cyclosporine A pretreatment can reduce the activity of LDH in myocardial cells after ischemia-reperfusion injury,reduce the CMAR and the activity of Na^＋,K^＋-ATP enzyme,and has a certain protective effect on myocardial ischemia-reperfusion injury.

关 键 词：环孢素A 乳酸脱氢酶 心肌细胞 缺血-再灌注损伤 Na+ K+-ATP酶 心脏灌注 离体大鼠心脏模型 

分 类 号：R965.2[医药卫生—药理学] R979.5[医药卫生—药学]