含七氟醚HTK液对心脏移植离体鼠心电生理的保护机制  

Protective Mechanism of containing sevoflurane HTK Solution on Cardiac Electrophysiology in Isolated Rat Cardiac Transplantation

作　　者：李伟超 高巨[2] 高鸿 佘玉琦 王子君 刘艳秋 LI Weichao;GAO Ju;GAO Hong;SHE Yuqi;WANG Zijun;LIU Yanqiu(Department of Anesthesia,Guizhou Medical University,Guizhou 550004,Chin)

机构地区：[1]贵州医科大学麻醉学院,贵州550004 [2]苏北人民医院,江苏扬州225001 [3]贵州医科大学附属医院麻醉科,贵州550004

出　　处：《实用医学杂志》2018年第9期1441-1444,共4页The Journal of Practical Medicine

基　　金：2017年度贵阳市人民政府-贵州医科大学科学技术联合基金资助项目(编号:201730-42);麻醉学专业学位研究生工作站基金资助项目(编号:080800818)

摘　　要：目的探讨含七氟醚HTK液对心脏移植离体鼠心电生理的保护机制。方法雄性SD大鼠24只,用Langendorff灌注装置建立离体大鼠心脏灌注和工作模型,分为对照组(C组,n=8)、七氟醚组(S组,n=8)、庚醇组(H组,n=8),三组心脏分别在HTK液、含七氟醚及含七氟醚+庚醇HTK液中保存心脏6 h。分别观察心脏复跳时间、心律失常发作时程、各时间点心率(HR)及心脏外层及内层的单相动作电位(MAP)并计算单相动作电位复极50%和复极90%时程(MAPD_(50),MAPD_(90))、0相最大上升速率(Vmax)、单相动作电位振幅(MAPA)。结果各组离体心脏均可成功复跳。与C组比较,S组和H组的心脏复跳时间明显缩短、T1时点的心率(HR)明显减慢、T1~2时内外膜MAPD50、MAPD90缩短、室颤发生率及再灌注心律失常评分均降低、室颤时程缩短(P<0.05)。三组各时点的V_(max)、MAPA无差异(P>0.05)。结论含七氟醚HTK液和含庚醇HTK液在电生理方面有相似作用,都可抑制延长的单相动作电位(MAP),减轻心律失常。含七氟醚HTK液的电生理保护作用可能与缝隙连接抑制相关。Objective To investigate the electrophysiological protective effect of HTK solution containingsevoflurane on rat cardiac transplantation. Methods Twenty-four male Sprague-Dawley rats were divided into thecontrol group,sevoflurane group and Heptanol group. Rat hearts in 3 groups were stored in HTK solution,contain-ing sevoflurane and sevoflurane ＋ heptanol HTK solution for 6 h. Heart resuscitation time,the duration of arrhyth-mia and monophasic action potential（MAP）and heart rate（HR）at different time points were recorded.Monophasicaction potential duration of repolarization at 50% and 90%（MAPD50 and MAPD90）,monophasic action potentialamplitude（MAPA）and maximal velocity（V_（max））were analyzed. Results The isolated rat hearts in each group canbe successfully restored to the spontaneous heart beat. Compared with group C,heart resuscitation time in group Sand group H was significantly shortened,heart rate（HR）was significantly decreased at T1,MAPD50 and MAPD90 in heart intima and epicardium were shortened at T1- T2,the incidence of ventricular fibrillation and reperfusionarrhythmia Scores were reduced,with the shorter duration of ventricular fibrillation（P 0.05）. No significant differ-ence was found in Vmax and MAPA among the three groups at each time point. Conclusion HTK solution contain-ing sevoflurane and HTK solution containing heptanol had similar electrophysiological effects,which could inhibitthe prolonged monophasic action potential（MAP）and reduce arrhythmia. The electrophysiological protective effectof HTK solution containing sevoflurane may be associated with the inhibition of gap junction function.

关 键 词：七氟醚 HTK液 心脏移植 单相动作电位 缝隙连接 再灌注心律失常 

分 类 号：R614[医药卫生—麻醉学]