Mg^(2+)对米曲霉HMP-F28所产α-CPA及β-CPA的调控作用  

Effect of Mg^(2+) on the Production of α-CPA, β-CPA in Aspergillus oryzae HMP-F28

作　　者：田小月 路旭[2,3] 王楠 胡江春[1,2] TIAN Xiao-yue;LU Xu;WANG Nan;HU Jlang-chun(Coll. of Pharm. Engin., Shenyang Pharm. Uni., Shenyang 110016;Inst. of Appl. Ecol., Chinese Acad. Sci., Shenyang 110016;Uni. Chinese Acad. Sci. , Beijing 100049)

机构地区：[1]沈阳药科大学制药工程学院,辽宁沈阳110016 [2]中国科学院沈阳应用生态研究所,辽宁沈阳110016 [3]中国科学院大学,北京100049

出　　处：《微生物学杂志》2018年第2期8-14,共7页Journal of Microbiology

基　　金：国家自然科学基金项目(31471814)

摘　　要：在米曲霉HMP-F28所产CPA(环匹阿尼酸)类化合物中,β-CPA是α-CPA的生物合成前体化合物,因此β-CPA的含量很低,而α-CPA是主要代谢产物。在研究HMP-F28所产CPA类代谢产物的调控过程中,采用SPE-LC-MS方法建立了一个对HMP-F28所产CPA类代谢物的快速定性分析方法。在此基础上,发现不同浓度的Mg^(2+)可调控α-CPA和β-CPA相对含量,高浓度Mg^(2+)(320 mmol/L)可使菌株显著提高生物合成中间体β-CPA的产量。由于β-CPA在结构上较α-CPA与IAA拮抗剂hypaphorine更类似,因此本研究为进一步研究其作为IAA拮抗剂诱导植物免疫反应的活性提供参考。Among CPAs（cyclopiazonic acids） produced by Aspergillus oryzae HMP-F28,β-CPA is a biosynthetic precursor of α-CPA,in consequence the content of β-CPA is very low,and α-CPA is the major metabolite. During the regulation and control process of CPAs production by HMP-F28,a SPE-LC-MS method was adopted to establish a rapid qualitative analysis for CPAs metabolite produced by HMP-F28. It was found based on these that different concentration of Mg^2＋could regulate and control the relative content of α-CPA and β-CPA,high concentration of Mg^2＋（320 mmol/L） could significantly increase the output of β-CPA. Since the structure of β-CPA is more similar to IAA antagonist hypaphorin,therefore,this study has laid a foundation to further study on β-CPA as IAA antagonist to induce the activity of plant immune responses.

关 键 词：环匹阿尼酸 IAA拮抗剂 米曲霉(Aspergillus oryzae)HMP-F28 SPE-LC-MS 

分 类 号：Q93-3[生物学—微生物学]