肺炎支原体肺炎患儿外周血及小鼠模型miRNAs差异表达  

Differential expression of miRNAs in children and mouse model with Mycoplasma pneumoniae pneumonia

作　　者：许长娣[1] 周瑶[1] 赵德育[1] 刘峰[1] Xu Changdi;Zhou Yao;Zhao Deyu(Dept of Respiratioo,Children’s Hospital of Nanjing Medical University,Nanjing 210008)

机构地区：[1]南京医科大学附属儿童医院呼吸科,南京210008

出　　处：《安徽医科大学学报》2018年第4期567-570,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(青年科学基金项目)(编号:81200012)

摘　　要：目的研究miRNAs在肺炎支原体肺炎患儿血中淋巴细胞以及在小鼠感染模型肺组织中的表达差异。方法通过miRNA芯片技术筛选24例肺炎支原体肺炎患儿和24例健康儿童血液淋巴细胞中差异表达的miRNAs,通过实时定量PCR法验证芯片检测结果的准确性,并构建支原体呼吸道感染小鼠模型,通过实时定量PCR法验证小鼠肺组织中相关miRNA的表达差异。结果在支原体肺炎患儿血液淋巴细胞中筛选出表达上调的miRNA 105个,下调的133个(P<0.05),通过PCR进一步证实患儿外周血及小鼠模型肺组织中miR-126、miR-181、miR-146a相对表达升高,miR-155相对表达降低,与芯片结果一致。结论 miR-126、miR-181、2017-12-18接收miR-146a、miR-155在肺炎支原体患儿外周血淋巴细胞及小鼠模型肺组织中存在差异表达,它们可能通过调控炎症因子的释放,参与肺炎支原体导致的免疫炎症反应。Objective To investigate the differential expression of miRNAs in blood lymphocytes in children with Mycoplasma pneumoniae pneumonia（ MPP） and lung tissue in mice models. Methods miRNAs chip technology was used to screen the miRNAs differentially expressed in 24 children with Mycoplasma pneumoniae pneumonia and24 healthy children. The results was verified by real-time quantitative PCR. Furthermore,in mice with Mycoplasma pneumoniae respiratory tract infection,quantitative PCR was used to validate the expression of miRNAs in the lung tissue. Results Compared with normal group,105 miRNAs increased and 133 decerased（ P〈0. 05） in MPP group. Among them,the relative expression of miR-126,miR-181 and miR-146 a increased and miR-155 decreased both in children peripheral blood and mice model lung tissue,validated by PCR,consistent with the results of the chip. Conclusion miR-126,miR-181,miR-146 a and miR-155 are differentially expressed in children peripheral blood lymphocytes and mouse lung tissues in MPP,which may be involved in the inflammation induced by Mycoplasma pneumoniae by regulating the release of inflammatory factors.

关 键 词：肺炎支原体 MIRNA 小鼠模型 

分 类 号：R725.6[医药卫生—儿科]