杞黄颗粒对年龄相关性黄斑变性患者血清补体的调控作用  

Effect of Qi-Huang granule on the serum level of CFH and C5b-9 in age-related macular degeneration patients

作　　者：王燕[1] 王璐[1] 袁远[1] 王莉[2] 黎红梅[2] 孟梁[2] 张欣桐[2] 梁凤鸣[2] WANG Yan;WANG Lu;YUAN Yuan;WANG Li;LI Hong - mei;MENG Liang;ZHANG Xin - tong;LIANG Feng - ming(Department of Ophthalmology, Guangdong Hospital of Traditional Chinese Medicine, Gaangzhou 510120, Guangdong, China.)

机构地区：[1]广东省中医院眼科,广东广州510120 [2]天津中医药大学第一附属医院眼科,天津300193

出　　处：《广东医学》2018年第7期1099-1102,1105,共5页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:81173308;81503618)

摘　　要：目的探讨杞黄颗粒对年龄相关性黄斑变性(AMD)患者血清补体因子H(CFH)及C5b-9的调控作用。方法采用临床随机对照试验研究设计方法,将AMD患者随机分为观察组(62例)和对照组(58例)。观察组口服杞黄颗粒,4.5 g/次,3次/d,对照组口服维生素C,0.2 g/次,3次/d,连续用药14周。采集AMD患者空腹静脉血并分离血清,采用ELISA法测定血清CFH及C5b-9蛋白质量浓度。结果观察组血清sCFH治疗前后比较差异均有统计学意义(P<0.01),但sC5b-9治疗前后比较差异无统计学意义(P>0.05)。对照组血清sCFH、sC5b-9治疗前后差异均无统计学意义(P>0.05)。两组之间治疗后sCFH浓度比较差异有统计学意义(P<0.05)。干性AMD患者观察组血清sCFH治疗前后差异均有统计学意义(P<0.05),但sC5b-9治疗前后差异均无统计学意义(P>0.05)。湿性AMD患者观察组血清CFH、C5b-9质量浓度治疗前后差异均无统计学意义(P>0.05)。结论杞黄颗粒对干性AMD患者的血清补体CFH有一定的调节作用,可能是中药杞黄颗粒可改善AMD患者视功能的作用机制之一。Objective The study aimed to evaluate the protective effects on the serum level of CFH and C5b -9 of Qi - Huang granule for age - related macular degeneration （AMD）. Methods This study was a case - control study. 120 AMD patients were randomly divided into experimental group （62 patients） who received Qi -Huang granule（ 10 g, rid） and control group（58 patients） who receieved Vitamin C （0. 2 g, tid）. After treatment of 3 months, the fasting blood of the patients was collected and the concentrations of serum CFH （ sCFH ） and C5b - 9 （ sC5b - 9 ） were measured by ELISA. Results Level of sCFH in the experimental group after the treatment was E 308. 25 （128.09） ] ng/mL, which was significantly higher than the baseline （ P 〈 O. 05 ）. Level of sCFH of the control group after the treatment was [ 246. 97 （ 158. 13 ） ] ng/mL higher than the baseline, but the difference was not statistically significant （P 〉 0. 05 ）. On the contra- ry, the expression of sC5b -9 in the experimental group after the treatment was E65.51 （47. 17） ] pg/mL, which was not statistically signific^int than the baseline （ P 〉 O. 05 ）. Level of sC5b - 9 of the control group after the treatment was [ 66. 45 （ 56. 33 ） ] pg/mL lower than the baseline, but the difference was not statistically significant （ P 〉 0. 05 ）. The plas- ma CFH concentration in the experimental group was higher than the baseline in dry AMD patients （P 〈 0. 05 ）, But there was no statistical significant difference in wet AMD patients The plasma C5b -9 concentrations were similar before and af- ter＇ treatment in both dry and wet AMD patients （ P 〉 0. 05 ）. Conclusion Our results revealed that Qi - Huang granule could increase the concentration of serum CFH in dry AMD patients. This indicates that Qi - Huang granule may have a protective role in the pathogenesis of.

关 键 词：年龄相关性黄斑病变 中药 杞黄颗粒 补体 CFH C5B-9 

分 类 号：R276.7[医药卫生—中医五官科学]