细粒棘球蚴重组蛋白GST诱导小鼠脾细胞增殖及对细胞因子水平的影响  

Changes of splenocyte lymphokines in mice induced by recombination protein EgGST(rEg.GST) against Echinococcus granulosus

作　　者：朱明星[1,2] 徐士梅 赵殷奇 牛楠[1,2] 赵巍[1,2] ZHU Ming - xing;XU Shi - mei;ZHAO Yin - qi;NIU Nan;ZHAO Wei(Center of Scientific Technology of Ningxia Medical University, Yin Chuan, Ningxia 750004, Chin)

机构地区：[1]宁夏医科大学科技中心,宁夏银川750004 [2]宁夏医科大学包虫病实验室,宁夏银川750004

出　　处：《现代预防医学》2018年第9期1667-1670,1678,共5页Modern Preventive Medicine

基　　金：国家自然科学基金(81360249);宁夏自然基金(NZ15210);宁夏医科大学校级科研项目(XM201407)

摘　　要：目的探讨细粒棘球蚴重组蛋白GST(rEg.GST)诱导小鼠产生免疫保护力的机制。方法将rEg.GST与弗氏佐剂混合免疫小鼠,腹腔注射原头蚴制备包虫感染模型,分离脾淋巴细胞,用rEg.GST和刀豆蛋白A(ConA)诱导后,四甲基偶氮唑盐比色法(MTT)检测小鼠脾淋巴细胞的增殖情况;酶联免疫吸附法(ELISA)检测培养上清液中白细胞介素-2(IL-2)、白细胞介素-4(IL-4)、γ-干扰素(IFN-γ)和白细胞介素-10(IL-10)的表达水平。结果重组蛋白免疫组在rEg.GST及Con A刺激状态下脾淋巴细胞增殖水平均高于佐剂组(P=0.003 0;P<0.001)和PBS组(P=0.001 4;P=0.006 1)。与PBS组比较,rEg.GST组在免疫后、感染后的IL-2、IFN-γ、与IL-4的水平均增高(P=0.007 7;P=0.041 9;P=0.014 8);rEg.GST组免疫后与免疫前相比,IL-2、IFN-γ、IL-4与IL-10水平均增高(P=0.001 7;P=0.023 1;P=0.003 1;P=0.021 2);感染后与免疫后相比,IL-4与IL-10水平升高,IL-2水平则呈下降趋势(P=0.009 4;P=0.039 3;P=0.0179)。结论 rEg.GST能够促进脾淋巴细胞的增殖并同时活化辅助性T细胞1型(Th1)与2型(Th2)细胞。Objective To investigate the immune-mechanism of recombinant Echinococcus granulosus glutathione S-transferase（rEg. GST） against the development of protoscolices（PSCs）. Methods Mice were immunized with rEg. GST and Freund＇s adjuvant,and splenic lymphocytes were aseptically isolated from mice infected with E. granulosus PSCs and induced by rEg. GST and Con A respectively or mixed induction. The proliferation of splenic lymphocytes was detected by MTT assay. The levels of interleukin-2（IL-2）,interleukin-4（IL-4）,γ-interferon（IFN-γ） and interleukin-10（IL-10） in the culture supernatants were detected by ELISA. Results The level of plenocyte proliferation in rEg. GST immune group were higher than control group after rEg. GST and Con A stimulation. After immunization and challenged with PSCs,the level of IL-2,IFN-γand IL-4 in the rEg. GST group were higher than those in the PBS group（P = 0. 007 7; P = 0. 041 9; P = 0. 014 8）.The levels of IL-2,IFN-γ,IL-4 and IL-10 in rEg. GST group after immunization were significantly higher than before（P= 0. 001 7; P = 0. 023 1; P = 0. 003 1; P = 0. 021 2）. The levels of IL-4 and IL-10 were significantly increased while IL-2 decreased after challenged with PSCs（P = 0. 009 4; P = 0. 039 3; P = 0. 017 9）. Conclusion Mice immunized with rEg. GST produce specific T cell immune response,it can activate Th1 and Th2 cell which play an important role to against Echinococcus granulosus.

关 键 词：细粒棘球蚴 rEg.GST TH细胞 细胞因子 

分 类 号：R113[医药卫生—公共卫生与预防医学]