陕西省87株耐药结核分枝杆菌寡核苷酸基因分型和耐药性分析  

Spoligotyping genotyping and drug resistance of 87 Mycobacterium tuberculosis strains,Shanxi

作　　者：李妍 曾小红[2] 杨健 王蕊 鲜小萍 张天华 LI Yan;ZENG Xiao -hong;YANG Jian;WANG Rui;XIAN Xiao -ping;ZHANG Tian -hua(Shaanxi Provincial Institute for TB Control and Prevention, Xi＇an, Shanxi 710048, China)

机构地区：[1]陕西省结核病防治研究所,陕西西安710048 [2]厦门大学生命科学学院,福建厦门361102

出　　处：《现代预防医学》2018年第9期1679-1683,共5页Modern Preventive Medicine

基　　金：陕西省社会发展科技攻关项目(No.2016SF-272)

摘　　要：目的对陕西87例耐药结核分枝杆菌进行分型研究,了解不同基因型在陕西的流行情况及耐药相关性。方法收集陕西省结核分枝杆菌耐药菌株87株,采用Spoligotyping方法对结核分枝杆菌临床分离株进行基因分型。结果 87株耐药结核分枝杆菌中北京家族占86.21%(75/87),非北京家族占13.79%(12/87);非北京基因型中,T家族(T1、T2、T2-3、T3)占9.20%(8/87),Manu家族占1.15%(1/87),新发现基因型占3.45%(3/87)。北京家族和非北京家族的单耐药、多耐药、耐多药、其他类型耐药比率分别为38.67%(29/75)、13.33%(10/75)、42.67%(32/75)、5.33%(4/75)和66.67%(6/9)、11.11%(1/9)、22.22%(2/9)、0.00(0/3),差异均无统计学意义(P>0.05)。结论陕西省结核分枝杆菌呈基因多态性,其主要流行基因型为北京家族;北京家族和非北京家族的耐药性差异无统计学意义。Objective To understand the prevalence of different genotypes and drug resistance through 87 clinically isolated drug-resistant Mycobacterium tuberculosiss（MTB） strains in Shanxi. Methods 89 clinically isolated drug-resistant MTB isolates were collected,whose genotypes were analyzed with Spoligotyping. Results 87 clinically isolated Mycobacterium tuberculosis strains mainly belonged to Beijing family（86. 21%,5/87） and the proportion of non-Beijing family was 13. 79%（12/87）. Among the non-Beijing family,8 isolates were part of T family（9. 20%,8/87）,including T1,T2,T2-3,T3 family,and 1 isolate was from the Manu family（1. 15%,1/87）. Additionally,3（3. 45%,3/87） isolations were new-found.The rates of MR-TB,PDR-TB,MDR-TB and other types of drug resistance in Beijing family and non-Beijing family were38. 67 %（29/75） vs 13. 33 %（10/75）,42. 67 %（32/75） vs 5. 33 %（4/75） and 66. 7 %（6/9） vs 11. 11 %（1/9）,22. 22 %（2/9） vs 0. 00（0/3）,respectively. All the resistance rates were not statistically significant（P〈 0. 05）. Conclusion Mycobacterium tuberculosis in Shanxi has genetic polymorphism and the main genotype is the Beijing family. There was no significant difference in the drug resistance between the Beijing family and non-Beijing family.

关 键 词：结核分枝杆菌 间隔区寡核苷酸分型 北京家族 耐药 

分 类 号：R115[医药卫生—公共卫生与预防医学]