基因分型结合血清学方法鉴定ABO血型亚型  

ABO blood subgroup identification with serological methods assisted by genotyping

作　　者：马晓莉[1] 马宏伟[1] 金新莉[1] 陈赞[1] 耿明璐[1] 张伯伟[1] MA Xiaoli;MA Hongwei;JIN Xin- li;CHEN Zhan;GENG Minglu;ZHANG Bowei.(Henan Red Cross Blood Center ,Henan,Zhengzhou 450000, China)

机构地区：[1]河南省红十字血液中心

出　　处：《中国输血杂志》2018年第2期151-153,共3页Chinese Journal of Blood Transfusion

基　　金：河南省医学科技攻关项目(201503199)

摘　　要：目的准确鉴定红细胞ABO亚型。方法采用血清学方法鉴定12例ABO血型正反定型不符标本,结合PCR-SSP基因定型方法作检测鉴定,并对其中9例作基因测序确定基因型。结果 ABO基因分型结果与血型血清检测结果一致率为66.67%（8/12）;其余不相符的4例：2例为B（A）,1例为Cis AB,1例为新基因（新基因血型血清学结果不确定,PCR-SSP结果为A1/O02,测序结果为A102/O02型）。结论 ABO血型基因分型技术结合血清学定型可用于ABO亚型鉴定及ABO血型的正确定型。Objective To identify the erythrocyte ABO subgroups accurately.Methods A total of 12 forward-reversetyping inconsistent sampleswere processedby serological methods Combined with PCR-SSP methods. 9 of the 12 samples were genotyped by DNA sequencing. Results The consistency rate between the ABO genotyping and serological results was66. 67%（ 8/12）; As for the 4 inconsistent samples： 2 cases were B（ A）,1 case was Cis AB and 1 case wasidentified as an unrecorded novelgenotype,whichcould not be clearly identified according tothe serological result whilethe PCR-SSPresult was A1/O02 andthe DNA sequencing result was A102/O02）. Conclusion ABO blood group genotyping technology combined with serological typing can be used for ABO subgroup identification,which promotes the test results in improvedaccuracyof ABO blood typing.

关 键 词：基因分型 ABO亚型 ABO血型基因 血型鉴定 血型血清学 

分 类 号：R457.11[医药卫生—治疗学] R446.62[医药卫生—临床医学]