小春花药材质量标准研究  

Study on Quality Standards for Sceptridium Ternatum

作　　者：辛文秀 钟里科[1] 张轶雯[1] 方罗[1] 郑小卫[1] 丁海樱[1] 黄萍[1,2] XIN Wenxiu;ZHONG Like;ZHANG Yiwen;FANG Luo;ZHENG Xiaowei;DING Haiying;HUANG Ping(Department of Pharmacy, Zhejiang Cancer Hospital, Hangzhou 310022, China;Key Laboratory of Head ＆ Neck Cancer Translational Research of Zhejiang Province, Hangzhou 310022, China)

机构地区：[1]浙江省肿瘤医院药剂科,杭州310022 [2]浙江省头颈肿瘤转化医学研究重点实验室,杭州310022

出　　处：《中国现代应用药学》2018年第4期542-546,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：浙江省中医药科技计划项目(2017ZB022);浙江省中医药重点项目(2016ZZ009);2016浙江省151第二层次培养项目;2016浙江省卫生创新人材培养项目

摘　　要：目的制定并考察小春花药材质量标准,为进一步开发小春花药材提供基础。方法采用紫外-可见分光光度法建立小春花中总黄酮的含量测定方法;采用HPLC同时测定小春花药材中槲皮素和山奈酚的含量。结果建立小春花总黄酮的测定方法,槲皮素为在30.23~181.40μg·m L^(-1)呈良好线性关系,R^2=0.998 4;确定药材中槲皮素、山奈酚的含量测定方法,槲皮素在2.64~21.12μg·m L^(-1)呈良好的线性关系,R^2=0.999 8,山奈酚在2.00~16.00μg·m L^(-1)呈良好的线性关系,R^2=0.999 9;小春花总黄酮以槲皮素计不得<0.2‰,山奈酚计不得<0.6‰。结论本研究对小春花药材进行质量控制,提升并建立了新的小春花药材质量标准。OBJECTIVE To establish the quality standards for Sceptridium ternatum,and lay the foundation for its further development.METHODS The content of total flavonoids was assayed by UV-Vis spectrophotometry.The contents of quercetin and kaempferol were determined by HPLC.RESULTS There was a good linear relationship between the absorbance values and the quercetin concentration when it was in the range of 30.23-181.40μg·m L-1（R2=0.998 4）.The quercetin and kaempferol were in good linear relationships in the range of 2.64-21.12μg·m L-1（R2=0.999 8）and 2.00-16.00μg·m L-1（R2=0.999 9）,respectively.The quercetin/kaempferol content in Sceptridium ternatum should not be less than 0.2‰and 0.6‰,respectively.CONCLUSION The quality control standard for Sceptridium ternatum is modified and a new one is provided to guarantee the quality of raw medical materials.

关 键 词：小春花 总黄酮 槲皮素 山奈酚 质量标准 

分 类 号：R284.1[医药卫生—中药学] R914.101[医药卫生—中医学]