鲜肤膏及其拆方治疗慢性湿疹的量效关系研究  

Dose-effect Relationship of Xianfu Ointment and its Decomposed Recipes on Chronic Eczema

作　　者：彭琳 穆玉雪 刘金玉 李晓亚[2] 葛莎莎 李爽 赵欣[2] 林余霖[2] 蔡大勇[2] 孙丽萍 唐炳华 刘连起 Peng Lin;Mu Yuxue;Liu Jinyu;Li Xiaoya;Ge Shasha;Li Shuang;Zhao Xin;Lin Yulin;Cai Dayong;Sun Liping;Tang Binghua;Liu Lianqi(College of Life Sciences,Beijing University of Chinese Medicine, Beijing 100029, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College)

机构地区：[1]北京中医药大学生命科学学院,北京100029 [2]中国医学科学院药用植物研究所

出　　处：《中国药师》2018年第5期817-823,共7页China Pharmacist

摘　　要：目的:探讨鲜肤膏及其拆方对2,4-二硝基氯苯(DNCB)诱导小鼠慢性湿疹的量效关系,确定半数有效剂量(ED_(50))及方剂配伍增效。方法:DNCB诱导C57小鼠慢性湿疹,给予梯度剂量鲜肤膏(11.71~11 662.50 mg·kg^(-1)·d^(-1),k=0.316)、地乌(0.53~530.12 mg·kg^(-1)·d^(-1),k=0.316)、鲜肤膏去地乌(11.18~11 132.40 mg·kg^(-1)·d^(-1),k=0.316)治疗,HE染色观察病理特点,ELISA检测血清白细胞介素2(IL-2)、干扰素-γ(IFN-γ)、白细胞介素4(IL-4)、白细胞介素13(IL-13)含量;Prism 5.0非线型可变斜率回归计算ED_(50),比较鲜肤膏、地乌、鲜肤膏去地乌组的药效,观察地乌的配伍增效作用。结果:鲜肤膏、地乌、鲜肤膏去地乌组药效具有剂量依赖特征,量效曲线呈"S"型。鲜肤膏组疗效最优,显著降低表皮厚度(ED_(50)=377.90mg·kg^(-1)·d^(-1)),减少真皮淋巴细胞浸润数(ED_(50)=153.20 mg·kg^(-1)·d^(-1)),增加血清IL-2(ED_(50)=608.90 mg·kg^(-1)·d^(-1))、IFN-γ(ED_(50)=205.50 mg·kg^(-1)·d^(-1))含量,降低血清IL-4(ED_(50)=198.70 mg·kg^(-1)·d^(-1))、IL-13(ED_(50)=117.60mg·kg^(-1)·d^(-1))含量。与鲜肤膏比较,地乌组与鲜肤膏去地乌组的量效曲线分别右移。结论:鲜肤膏及其拆方均能有效治疗湿疹;地乌有配伍增效作用,鲜肤膏组的疗效最优。Objective： To investigate the dose-effect relationship of Xianfu ointment and its decomposed recipes the l-chloro-2,4-dini-trochlorobenzene（DNCB） induced chronic eczema in mice, and confirm the median effective dose （ED50） of each formula and the synergetic effect by compatibility. Methods： DNCB was used to induce chronic eczema in C57 mice. The mice were treated with gradient dosages of the Xianfu ointment （11.71-11 662.50 mg ·kg-1 · d-1 ,k = 0.316）, Anemone flaccid （0.53-530.12 mg· kg-1· d-1,k = 0.316）, Xianfu ointment without Anemone flaccid （11.18-11 132.40 mg· kg-1· d-1 ,k = 0.316）, respectively. The pathological features were observed after hematoxylin-eosin staining. The volume ratio of epidermides and the number of lymphocyte infiltrated in dermis were analyzed with morphometry. The serum levels of IL-2, IFN-γ, IL-4, and IL-13 were detected by ELISA assay. The ED50 was calculated by non-linear regression with various slope using Prism-5.0 software. Results： The effects of Xianfu ointment and its decomposed recipes on chronic eczema showed a dose-dependent tendency. The dose-response curves showed ＂S＂ shape. The efficacy of Xianfu ointment on chronic eczema was the most significant among the three formulas, which was demonstrated by decreased epidemical thicknes （EDs0 = 377.90 mg· kg-1· d-1~）, reduced infiltrated lymphocyte number（ED5o = 153.20 mg· kg-1· d-1） , increased serum IL-2（ED50 = 608.90 mg· kg-1 · d-1） and IFN-γ （ED50 = 205.50 mg ·kg-1·d-1） levels, and decreased serum IL-4（ED50 = 198.70 mg · kg-1· d-1） and IL-13 levels （ED50 = 117.60 mg·kg-1· d-1）. And the dose-effect curves of Anemone flaccid and Xianfu ointment without Anemone flaccid groups were both right shift when compared with that of Xianfu ointment. Conclusion： Xianfu ointment and its decomposed recipes can effectively treat chronic eczema. Anemone flaccid has obvious compatibility synergy in the whole formula. The effects of Xianfu ointment is most significant.

关 键 词：慢性湿疹 鲜肤膏 量效关系 免疫调节 配伍增效 

分 类 号：R285.5[医药卫生—中药学]