连续高强度间歇运动对中年久坐健康男性外周血淋巴细胞凋亡的影响  

Effect of Consecutive High Intensity Intermittent Exercise on Peripheral Blood Lymphocyte Apoptosis in Healthy Sedentary Middle-Aged Men

作　　者：刘鸣[1] 夏盈盈[1] 张淑兰 宋琳[3] 张新[3] 朱荣[4] LIU Ming;XIA Ying-ying;ZHANG Shu-lan;SONG Lin;ZHANG Xin;ZHU Rong(Ningbo City College of Vocational Technology, Ningbo 315100, Zhejiang China;Beijing Gucheng Middle School, Beijing 100043, China;Beijing Sport University, Beijing 100084, China;Wezhou Medical University, Wenzhou 325035, Zhejiang China)

机构地区：[1]宁波城市职业技术学院,浙江宁波315100 [2]北京市古城中学,北京100043 [3]北京体育大学,北京100084 [4]温州医科大学,浙江温州325035

出　　处：《北京体育大学学报》2018年第3期68-75,共8页Journal of Beijing Sport University

基　　金：浙江省教育厅科研计划资助项目(编号:Y201017123)

摘　　要：目的:研究连续3 d高强度间歇运动(HIIE)和中等强度持续运动(MICE)对外周血淋巴细胞凋亡的影响并探讨其可能机制。方法:16名中年久坐健康男性分别进行连续3 d HIIE(30 s全力蹬车+4 min间歇,×4组)和MICE(60%6)VO2max持续蹬车40 min),于运动前(pre)、第1 d运动后3 h(post1)、第2 d运动后3 h(post2)、第3 d运动后3 h(post3)和24 h(post24)取静脉血测定淋巴细胞计数、淋巴细胞凋亡率、淋巴细胞线粒体膜电位(MTP)、超氧阴离子、Caspase-3活性、Bcl-2蛋白表达以及血清可溶性Fas配体(s Fas L)、白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)、C-反应蛋白(CRP)和皮质醇(CRT)含量。将新鲜淋巴细胞分别与HIIE-pre血清、HIIE-post3血清、糖皮质激素受体阻断剂米非司酮(MIF)预处理的HIIEpost3血清以及单一介质(分别为L-乳酸、hr IL-6、hr CRP和氢化可的松)在培养基中共孵育24 h,用流式细胞仪观察淋巴细胞凋亡率的变化。结果:淋巴细胞计数及比例在HIIE-post1,MTP和Bcl-2蛋白表达量在post2开始降低(P<0.05),post3达到谷值(P<0.05);淋巴细胞超氧阴离子在HIIE-post1,淋巴细胞凋亡率、Caspase-3活性和血清CRT在post2开始升高(P<0.05),post3达峰值(P<0.05);血清IL-6和CRP在HIIE和MICE后呈脉冲式升高(P<0.05),且HIIE升高幅度低于MICE(P<0.05);HIIE-post24后除Bcl-2蛋白表达仍高于pre(P<0.05)外,其他各指标均恢复(P>0.05)。HIIE-post3血清与淋巴细胞共孵育后细胞凋亡率明显高于pre血清与淋巴细胞共孵育(P<0.05)。单一血清介质与淋巴细胞共孵育显示,L-乳酸、hr IL-6和hr CRP对淋巴细胞凋亡率均无显著性影响(P>0.05),氢化可的松则上调淋巴细胞凋亡率(P<0.05),加入MIF后淋巴细胞凋亡率显著降低(P<0.05)。结论:1)连续3 d HIIE可诱导中年久坐健康男性外周血淋巴细胞凋亡,建议连续HIIE训练2 d后进行适当调整;2)线粒体氧化应激和皮质醇-糖皮质激素受体信号途径是连续HIIE诱导外周血淋Objective： This study aimed at exploring the effect of 3 consecutive days high intensity intermittent exercise（ HIIE） and moderate intensity continuous exercise（ MICE） on peripheral blood lymphocyte apoptosis and investigating the possible mechanism. Methods： Sixteen healthy sedentary middle-aged men performed 3 consecutive days HIIE（ 4 × 30 s cycling,interspersed with 4 min rest） and MICE（ 40 min continuous cycling at 60%6）VO2 max） respectively. Venous blood was taken and parameters of lymphocyte count,lymphocyte apoptosis rate,lymphocyte mitochondrial transmembrane potential（ MTP）,superoxide,Caspase-3 activity,Bcl-2 protein expression,and serum soluble Fas ligand（ s Fas L）,interleukin-6（ IL-6）,tumor necrosis factor-α（ TNF-α）,C-reaction protein（ CRP） and corticosteroid（ CRT） were measured before exercise（ pre）,3 h after the 1 st day exercise（ post1）,3 h after the 2 nd day exercise（ post2）,3 h（ post3） and 24 h（ post24） after the 3 rd day exercise respectively. Fresh lymphocytes and HIIE-pre serum,HIIE-post3 serum,HIIE-post3 serum preconditioning of glucocorticoid receptor blocking agent mifepristone（ MIF）,single mediator（ L-Lactic acid,hr IL-6,hr CRP and hydrocortisone） respectively were incubated in culture medium for 24 h,and the change of lymphocyte apoptosis apoptotic rate was determined by flow cytometry. Results： Lymphocyte count and lymphocyte proportion at HIIEpost1 and MTP and Bcl-2 protein expression at post2 began to reduce（ P〈0. 05）,and reached the minimum level at post3（ P〈0. 05）. Lymphocyte superoxide at HIIE-post1,lymphocyte apoptosis rate,Caspase-3 activity and serum CRT at post2 increased and reached the peak level at post3（ P〈0. 05）. Serum IL-6 and CRP of HIIE and MICE increased with pulsed-wave mode（ P〈0. 05）,and the increase of HIIE was less than MICE（ P〈0. 05）. At HIIE-post24,all indicators were restored to pre-exercise（ P〈0. 05）,except that Bcl-2 protein was still higher（ P〈

关 键 词：高强度间歇运动 中等强度持续运动 淋巴细胞凋亡 线粒体 皮质醇 糖皮质激素受体 

分 类 号：G804.2[文化科学—运动人体科学]