卡介苗干预对IL-4诱导的巨噬细胞炎症细胞因子和粘附分子表达的影响  

Influence of Bacille Calmette-Guerin vaccine intervention on the expression of macrophage inflammatory cytokines and adhesion molecules in IL-4 induction

作　　者：伍雪梅[1] 刘丽敏[1] 张丽[1] WU Xue Mei;LIU Li Min;ZHANG Li(Department of Gynecology,Maternal and Child Health Hospital of Shenzhen City, Guangdong, .518000, China)

机构地区：[1]广东省深圳市妇幼保健院妇科,518000

出　　处：《中国妇幼卫生杂志》2018年第1期27-30,共4页Chinese Journal of Women and Children Health

基　　金：深圳市科技创新委员会基础研究课题项目(JCYJ20160427190929616)-卡介苗(BCG)干预对宫颈癌微环境中巨噬细胞极化与炎癌关系变化的机制研究

摘　　要：目的探究卡介苗(BCG)干预对IL-4诱导的巨噬细胞炎症细胞因子(IL-12、IL-6、IL-10、MRC-1)和粘附分子(CCL-2)表达的影响。方法应用PMA(10 ng/m L)与IL-4(10 ng/m L)刺激巨噬细胞,使其向M2转型,并将溶于DMSO的0 mg/L、20 mg/L、50 mg/L及100 mg/L的BCG作用24 h,应用PCR的方法测定各组中IL-12、IL-6、IL-10、MRC-1及CCL-2 mRNA的表达。结果 IL-4刺激后IL-12、IL-6、IL-10、MRC-1及CCL-2 mRNA含量均有显著升高(P<0.01),而给予不同浓度的BCG后,IL-12、IL-6、IL-10、MRC-1及CCL-2 mRNA含量均有显著下降(P<0.01),100 mg/L的BCG作用最为明显。结论卡介苗能够抑制IL-4刺激的巨噬细胞炎症细胞因子和粘附分子的表达。Objective To explore the influence of Bacille Calmette-Guerin（BCG） vaccine intervention on the expression of macrophage inflammatory cytokines and adhesion molecules in IL-4 induction. Methods Macrophages were stimulated with PMA（10 ng/ml） and IL-4（10 ng/ml）,and transformed into M2. Then they were dissolved at DMSO 0 mg/L,20 mg/L,50 mg/L and 100 mg/L BCG for 24 hours. The expressions of IL-12,IL-6,IL-10,MRC-1 and CCL-2 mRNA in each group were determined by PCR methods. Results After IL-4 stimulation,the contents of IL-12,IL-6,IL-10,MRC-1 and CCL-2 mRNA were increased significantly（P〈0. 01）. After dissolved in BCG,the contents of IL-12,IL-6,IL-10,MRC-1 and CCL-2 mRNA were decreased significantly（P〈0. 01）. It was more significant after dissolved at 100 mg/L BCG. Conclusion The BCG vaccine can inhibit the expression of the macrophage inflammatory cytokines（IL-12,IL-6,IL-10,MRC-1） and Adhesion molecule（CCL-2） which were stimulated by IL-4.

关 键 词：卡介苗 巨噬细胞 炎症因子 粘附因子 IL-4 

分 类 号：R392[医药卫生—免疫学]