不同糖及糖水平对松浦镜鲤养殖过程中糖代谢相关基因表达的影响  

Effects of different carbohydrates and different carbohydrate levels on mRNA expression of glucose metabolism related genes in different feeding stages of Songpu mirror carp(Cyprinus carpio)

作　　者：李晋南[1] 王常安[1] 王连生[1] 赵志刚[1] 罗亮[1] 徐奇友[1] LI Jinnan;WANG Chang＇an;WANG Liansheng;ZHAO Zhigang;LUO Liang;XU Qiyou(Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China)

机构地区：[1]中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨150070

出　　处：《水产学报》2018年第5期766-776,共11页Journal of Fisheries of China

基　　金：中央级公益性科研院所基本科研业务费专项(HSY201605);国家自然科学基金(31702353);现代农业产业技术体系建设专项(CARS-45)~~

摘　　要：为探讨在养殖不同阶段,不同糖及糖水平对松浦镜鲤肝脏糖酵解酶(GK和HK)、糖异生酶(G6Pase和PEPCK)和糖代谢相关基因(IGF-Ⅰ和GHR)m RNA表达水平的影响,实验采用2×2双因素设计实验,选择淀粉和葡萄糖2种糖源,2个糖水平(25%和50%),共4个实验组,分别为低淀粉组(LS)、高淀粉组(HS)、低葡萄糖组(LG)和高葡萄糖组(HG)。选用初始体质量为(8.30±0.15)g的松浦镜鲤420尾,随机分为4组,每组3个重复,每个重复35尾鱼。实验周期为7周。结果显示,不同养殖阶段,HK基因和IGF-Ⅰ基因在不同实验组中的表达趋势基本一致,HK基因在养殖1周时表达量显著升高,之后开始降低,IGF-Ⅰ基因在养殖3周时表达量显著升高。在不同养殖阶段,GK、PEPCK、G6Pase和GHR基因的表达趋势受糖水平和糖种类的影响。在低糖水平下(LS组和LG组),这些基因表现了相同的变化趋势,GK和GHR基因在1周时表达量最高,之后开始下降;PEPCK基因在3周时表达量最高;G6Pase在1周时表达量升高,之后下降,在7周时表达量又有上升趋势。在高糖水平下,HS组的GK基因的表达量在各个养殖阶段差异不显著,而HG组的GK基因则在1周时表达量显著升高;HS组和HG组的PEPCK基因的表达量在各个养殖阶段差异不显著;HS组的G6Pase基因的表达量在各个养殖阶段差异不显著,而HG组的G6Pase基因则在3周时表达量显著升高;HG组的GHR基因的表达量在各个养殖阶段差异不显著,而HS组的GHR基因则在3周时表达量显著升高。在养殖初期(1周),高糖饲料显著促进了HK基因的表达,并抑制了PEPCK、G6Pase和GHR基因的表达。This experiment was aimed to study the effect of different carbohydrates and different carbohydrate levels on m RNA expression of glycolytic enzyme（glucose kinase, GK; hexokinase, HK）, gluconeogenesis enzyme（Glucose-6-phosphatase, G6 Pase; phosphoenolpyruvate carboxykinase, PEPCK）, glucose metabolism related genes（Insulin-like Growth Factor-Ⅰ, IGF-Ⅰ; Growth Hormone Receptor, GHR） in different feeding stages of Songpu mirror carp（Cyprinus carpio）. Four experimental diets were formulated to contain two carbohydrates（starch and glucose） diets with two levels（250 g/kg and 500 g/kg）（Low level starch group： LS; High level starch group： HS; Low level glucose group： LG; High level glucose group： HG） two carbohydrates（glucose and starch）and two carbohydrate levels（25% and 50%）（LS/HS/LG/HG） using 2×2 double factor experimental design, 420 Songpu mirror carp（mean weight=[8.30±0.15] g） were randomly divided into 4 dietary treatments with 3 triplicates and 35 fish per triplicate and were reared for 7 weeks. The results showed that in different feeding stages, the m RNA expression levels of HK gene and IGF-Ⅰ gene in different experimental groups were basically in the same trend. The m RNA expression levels of HK gene at feeding 1 W was significantly increased and then decreased. The m RNA expression level of IGF-Ⅰ gene was significantly increased at feeding 3 W. IGF-Ⅰ gene expression increased significantly at feeding 3 W. In different feeding stages, the m RNA expression levels of GK, PEPCK,G6 Pase and GHR genes were affected by carbohydrate levels and carbohydrate types. At low carbohydrate level（LS group and LG group）, the m RNA expression levels of these genes showed the same trend. The m RNA expression levels of GK and GHR genes were significantly higher at feeding 1 W and then began to decline. The m RNA expression level of PEPCK gene was the highest at feeding 3 W. The m RNA expression level of G6 Pase genes were significantly higher at feeding 1 W, the

关 键 词：松浦镜鲤 葡萄糖 淀粉 糖酵解酶 糖异生酶 基因表达 

分 类 号：Q785[生物学—分子生物学] S963.7[农业科学—水产养殖]