自噬在富氢液减轻UVB致皮肤成纤维细胞损伤中的作用  

Hydrogen Alleviated Cellular Injury in Human Fibroblast Induced by UVB Irradiation Via Activating Autophagy

作　　者：赵子申 高雅丽[2] 张群 刘婕 刘文芳[3] 李秀芬 付国俊 陈红光[5] ZHAO Zishen;GAO Yali;ZHANG Qun;LIU Jie;LIU Wenfang;LI Xiufen;FU Guojun;CHEN Hongguang(Department of Dermatology, Cangzhou People＇s Hospital, Cangzhou 061000, China;Cangzhou Central Hospital, Cangzhou 061001, China;Cangzhou Medical College, Cangzhou 061001, China;Out-patient Operating Room, Cangzhou People＇s Hospital, Cangzhou 061000, China;Tianjin Institute of Anesthesiology, General Hospital of Tianjin Medical University, Tianjin 300052, China)

机构地区：[1]沧州市人民医院皮肤科,河北沧州061000 [2]沧州市中心医院,河北沧州061001 [3]河北省沧州医学高等专科学校,河北沧州061000 [4]沧州市人民医院门诊手术室,河北沧州061000 [5]天津医科大学总医院,天津300052

出　　处：《中国皮肤性病学杂志》2018年第5期507-512,共6页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金面上项目(81471842);沧州市科技计划项目(162302093)

摘　　要：目的探讨自噬对富氢液减轻UVB照射导致的人皮肤成纤维细胞氧化损伤的调控作用。方法取健康男童包皮环切术后包皮,进行人真皮成纤维细胞的原代培养,将细胞分组:正常对照组(Con组);氢气对照组(Con+H_2);UVB照射组(UV组);UVB照射+富氢培养液组(UV+H_2组);UVB照射+富氢培养液+自噬抑制剂3-MA组(UV+H_2+3-MA组),各组培养6h、12h和24h,电镜观察自噬体和LC3的表达变化,根据上述结果,各组在UVB照射后培养6h后,进行相MTT、LDH、ROS、MDA和自噬蛋白Beclin-1、ATG5和P62的检测。结果与Con组和Con+H_2组比较,UV组MTT值和P62表达减少(P<0.05),自噬体、LDH、ROS、MDA、LC3Ⅱ/Ⅰ、Beclin-1和ATG5表达增加(P<0.05);与UV组比较,UV+H_2组自噬体、MTT、LC3Ⅱ/Ⅰ、Beclin-1和ATG5表达增加(P<0.05),LDH、ROS、MDA和P62表达减少(P<0.05);与UV+H_2组比较,UV+H_2+3-MA组自噬体、MTT、LC3Ⅱ/Ⅰ、Beclin-1和ATG5表达减少(P<0.05),LDH、ROS、MDA和P62表达增加(P<0.05)。结论富氢液通过激活自噬途径减轻UVB照射导致的人皮肤成纤维细胞损伤。Objective To investigate the regulatory role of autophagy in alleviating cellular oxi- dative injury by hydrogen in human fibroblast that induced by UVB irradia- tion. Methods Dermal fibroblasts were isolated from the circumcised foreskin of healthy boys to manage the primary culture, which were then divided into five groups： control group （ Con group）, control ＋ H2 group （ Con ＋ H2 group）, UVB irradiation group （ UV group）, UVB irradiation ＋ H2 group （ UV ＋ H2 group）, and UVB irradia- tion ＋ H2 ＋ autophagy inhibitor 3-MA group （ UV ＋ H2 ＋ 3-MA group）. The autopha- gosome and LC3 expression were detected at 6h, 12h and 24h after UV irradiation in different groups, and on the basis of the results above, MTY, LDH, ROS, MDA au- tophagosome and autophagy proteins Beclin-1, ATG5 and P62 were detected at 6h af- ter UV irradiation in different groups. Results Compared with Con group and Con ＋ H2 group, MTT and P62 reduced, autophagosome, ROS, MDA, LC3 Ⅱ/Ⅰ , Beclin- 1 and ATG5 were increased in UV group （P 〈 0.05 ） ; compared with UV group, au- tophagosome, MTY, LC3 Ⅱ/Ⅰ, Beclin-1 and ATG5 increased, LDH, ROS, MDA and P62 reduced in UV ＋ H2 group （P 〈 0. 05） ; compared with UV ＋ H2 group, au- tophagosome, MTY, LC3 Ⅱ/ Ⅰ, Beclin-1 and ATG5 were increased（ P 〈 0. 05 ） , and LDH, ROS, MDA and P62 were elevated in UV ＋ H2 ＋ 3-MA group （P 〈 0. 05）. Conclusion Hydrogen-rich culture alleviated the cellular injury via activating auto- phagy in human fibroblast induced by UVB irradiation.

关 键 词：UVB 细胞损伤 氢分子 自噬 成纤维细胞 

分 类 号：R751[医药卫生—皮肤病学与性病学]